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| 柔嫩艾美耳球虫TA4抗原基因cDNA在乳酸乳球菌中的克隆与表达 | |
| 引用本文： | 张莉,秦泽荣,崔尚金,何召庆,刘尚高.柔嫩艾美耳球虫TA4抗原基因cDNA在乳酸乳球菌中的克隆与表达[J].中国预防兽医学报,2006,28(3):271-274. |
| 作者姓名： | 张莉 秦泽荣 崔尚金 何召庆 刘尚高 |
| 作者单位： | 1. 北京市农林科学院,畜牧兽医研究所,北京,100089 2. 中国农业大学,动物医学院,北京,100094 3. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001 |
| 摘 要： | 用PCR技术特异扩增了柔嫩史美耳球虫TA4抗原基因cDNA序列，克隆至质粒pMD18-T中，获得重组质粒pMD18-T-TA4，采用KpnⅠ／SacⅠ双酶切法及PCR确认正确后，将TA4基NcDNA目的片段亚克隆到乳酸乳球菌表达载体pMG36n中，电穿孔法转化乳酸乳球菌LM0230，获得重组质粒pMG36n-TA4，采用KpnⅠ／SacⅠ双酶切法及DNA测序证明cDNA序列完全正确。获得TA4乳酸乳球菌表达株，经SDS—PAGE电泳分析，结果表明表达产物与预期大小的TA4蛋白分子量一致。 |
| 关 键 词： | 柔嫩艾美耳球虫 TA4抗原基因cDNA 乳酸乳球菌 克隆与表达 |
| 文章编号： | 1008-0589(2006)02-0271-04 |
| 收稿时间： | 2005-04-13 |
| 修稿时间： | 2005年4月13日 |
Cloning and expression of TA4 antigen from Eimeria tenella in Lactococcus lactis |
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| ZHANG Li,QIN Ze-rong,CUI Shang-jin,HE Zhao-qing,LIU Shang-gao.Cloning and expression of TA4 antigen from Eimeria tenella in Lactococcus lactis[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(3):271-274. | |
| Authors: | ZHANG Li QIN Ze-rong CUI Shang-jin HE Zhao-qing LIU Shang-gao |
| Institution: | 1. Beijing Municipal Academy of Agriculture and Forestry, Beijing 100089, China; 2. China Agricultural University Beijing 100094, China; 3. National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin 150001, China |
| Abstract: | TA4 antigen cDNA of E.tenella was amplified by PCR and cloned into a vector pMD18-T.The positive plasmid contained TA4 antigen cDNA was determinded by restriction enzyme analysis and PCR.The plasmid pMD18-T-TA4 and the vector pMG36n were both digested by Sac I and Kpn I.The cDNA encoding TA4 antigen was subcloned into pMG36n and transferred into Lactococcus lactis LM0230 by electroporation.It proved that the positive recombinant plasmid pMG36n-TA4 contained cDNA encoding TA4 antigen by restriction enzyme analysis and sequence determination.In the positive transformants,the TA4 antigen was expressed as a fusion protein in Lactococcus lactis LM0230,which was verified by SDS-PAGE. |
| Keywords: | EimeHa tenella TA4 antigen Lactococcus lactis cloning and expression |
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