| 黄瓜序列特征性扩增区域标记(SCAR)的开发 |
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| 引用本文： | 李效尊,袁晓君,蒋苏,杜辉,潘俊松,何欢乐,吴爱忠,蔡润.黄瓜序列特征性扩增区域标记(SCAR)的开发[J].分子植物育种,2007,5(3):393-402. |
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| 作者姓名： | 李效尊 袁晓君 蒋苏 杜辉 潘俊松 何欢乐 吴爱忠 蔡润 |
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| 作者单位： | 上海交通大学农业与生物学院,上海,201101 |
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| 基金项目： | 上海市自然科学基金
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上海市科委资助项目 |
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| 摘 要： | 黄瓜的分子标记连锁图谱研究已经到了比较和整合的阶段,然而可用于黄瓜作图的锚定标记却并不多.本研究利用华北类型和欧洲温室型黄瓜自交系S94和S06作为PCR模板,通过将二者差异的RAPD和SRAP条带克隆、测序并根据测序结果设计特异引物,成功获得了118个SCAR标记.经4个典型黄瓜种质材料PCR验证,引物多态性比例高达10%以上.本研究获得的SCAR可望用于黄瓜遗传图谱的构建和整合.
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| 关 键 词： | 黄瓜 分子标记 SCAR |
Development of Sequence Characterized Amplified Region(SCAR) Markers in Cucumber |
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| Li Xiaozun,Yuan Xiaojun,Jiang Su,Du Hui,Pan Junsong,He Huanle,Wu Aizhong,Cai Run.Development of Sequence Characterized Amplified Region(SCAR) Markers in Cucumber[J].Molecular Plant Breeding,2007,5(3):393-402. |
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| Authors: | Li Xiaozun Yuan Xiaojun Jiang Su Du Hui Pan Junsong He Huanle Wu Aizhong Cai Run |
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| Abstract: | It would be the time for integration of molecular linkage maps in cucumber, but there is little of the anchor markers for using map integration. In this study, North China type inbred line S94 and Europe Greenhouse type inbred line S06 were used as PCR DNA template denotes. Totally 118 SCAR markers have been obtained from special primers designed by the sequences of RAPD and SRAP polymorphic bands between S94 and S06. These SCAR markers obtained in this research could be at least 10% polymorphic primers validated among the four typical cucumber germplasms, which would be used to constructing the molecular linkage map in cucumber. |
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| Keywords: | Cucumber Molecular marker SCAR |
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