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A novel fluorescent pH probe for expression in plants |
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| Authors: | Alexander Schulte Inken Lorenzen Markus Böttcher Christoph Plieth |
| Institution: | 1. Zentrum für Biochemie und Molekularbiologie, Universit?t Kiel, Am Botanischen Garten 9, 24118, Kiel, Germany 2. Biochemisches Institut, Universit?t Kiel, Rudolf-H?ber-Str. 1, 24098, Kiel, Germany 3. Botanisches Institut, Universit?t Kiel, Am Botanischen Garten 9, 24118, Kiel, Germany 4. Institut für Physiologie, Universit?t zu Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany |
| Abstract: | BackgroundThe pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue for monitoring cellular pH. They are derived from the most popular Aequorea victoria GFP (Av-GFP). Here, we present a novel fluorescent pH reporter protein from the orange seapen Ptilosarcus gurneyi (Pt-GFP) and compare its properties with pHluorins for expression and use in plants. |
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