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| 一种快速高效适于橡胶树叶片AFLP分析的DNA提取方法 | |
| 引用本文： | 钟淦彬,李维国,郑学项,李海林,颜园园,易晓洁,陈祖兴.一种快速高效适于橡胶树叶片AFLP分析的DNA提取方法[J].热带农业科学,2010,30(5):1-5. |
| 作者姓名： | 钟淦彬 李维国 郑学项 李海林 颜园园 易晓洁 陈祖兴 |
| 作者单位： | 1. 中国热带农业科学院橡胶研究所,海南儋州,571737;海南大学农学院,海南儋州,571737 2. 中国热带农业科学院橡胶研究所,海南儋州,571737 3. 中国热带农业科学院橡胶研究所,海南儋州,571737;海南大学环境与植物保护学院,海南儋州,571737 |
| 基金项目： | "十一五"国家科技支撑计划，植物新品种DUS测试指南研制项目 |
| 摘 要： | 以近200个巴西橡胶树种质材料（品种及无性系）幼嫩叶片为材料．采用改良的cTAB法对其基因组总DNA的提取进行了研究。结果表明，该方法提取的DNA溶液无色透明，DD260／DD280的比值均为1．8～2．0，1％琼脂糖凝胶电泳为清晰的一条带，无降解现象，KNA去除干净，能被限制性内切酶完全消化；其AFLP分析（引物EcoRI-TA／MseI—CTT）条带清晰，多态性好，说明此方法提取的巴西橡胶树幼嫩叶片基因组总DNA较适于AFLP分析。 |
| 关 键 词： | 巴西橡胶树 AFLP分析 DNA提取 |
A Simple and Effective DNA Extraction Method for AFLP Analysis in Hevea brasiliensis Muell Arg. |
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| ZHONG Ganbin,LI Weiguo,ZHENG Xuexiang,LI Hailin,YAN Yuanyuan,YI Xiaojie,CHEN Zuxing.A Simple and Effective DNA Extraction Method for AFLP Analysis in Hevea brasiliensis Muell Arg.[J].Chinese Journal of Tropical Agriculture,2010,30(5):1-5. | |
| Authors: | ZHONG Ganbin LI Weiguo ZHENG Xuexiang LI Hailin YAN Yuanyuan YI Xiaojie CHEN Zuxing |
| Institution: | 1. Rubber Research Institute, CATAS, Danzhou, Hainan 571737; 2 College of Agronomy, Hainan University, Danzhou, Hainan 571737;3 Environment and Plant Protection Department of Hainan University, Danzhou, Hainan 571737)) |
| Abstract: | The genomic DNA samples of tender leaves of approximately two hundred germplasms of Hevea brasiliensis Muell. Arg. （varieties and clones） were isolated by improved CTAB method. The results showed that the extracted DNA of mature leaves by this method was pure and integral. The value of OD260/OD280 was 1.8 to 2.0. Degradation was nearly free. The RNA was eliminated completely, and suitable for digestion by restriction endonucleases. AFLP analysis indicated the primer （EcoRI-TA/MseI- CTT） produced clear polymorphic patterns. Therefore this method could be used for extracting ideal DNA samples from tender leaves of Hevea brasiliensis Muell. Arg. for AFLP analysis. |
| Keywords: | Hevea brasiliensis Muell Arg AFLP analysis DNA extraction |
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