A simple method for long-term cryopreservation of <Emphasis Type="Italic">Calonectria ilicicola</Emphasis> on barley grains |
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| Authors: | Sunao Ochi Akio Nakagawa |
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| Institution: | (1) National Agriculture Research Center, 3-1-1 Kannondai, Tsukuba Ibaraki, 305-8666, Japan |
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| Abstract: | We developed a simple method for long-term preservation of the soybean red crown rot fungus, Calonectria ilicicola, using barley grains. Autoclaved barley grains were inoculated with the fungus, then incubated at 25°C for 1 month. After
incubation, grains were dried to approximately 3% moisture content, and stored at 4, −20, or −80°C for 3 years. C. ilicicola preserved on barley grains at −80°C remained viable without any change in mycelial growth and virulence. These results showed
that C. ilicicola can be successfully cryopreserved for extended periods on barley grains at −80°C. We also confirmed that cultures preserved
on barley grains are suitable for direct use without further manipulation as inocula in pathogenicity tests. |
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