| 稻黑孢菌生物学特性及杀菌剂筛选 |
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| 引用本文： | 李戌清,傅鸿妃,李红斌.稻黑孢菌生物学特性及杀菌剂筛选[J].长江蔬菜,2016(6). |
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| 作者姓名： | 李戌清 傅鸿妃 李红斌 |
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| 作者单位： | 杭州市农业科学研究院,310024 |
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| 基金项目： | 杭州市科委资助项目(20140432B10) |
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| 摘 要： | 研究了稻黑孢菌的生物学特性,并对防治药剂进行了室内筛选。试验结果表明,菌株HZ4111菌丝生长的最适条件为温度30℃、12 h光12 h暗的交替光周期、培养基p H值5.0;在以可溶性淀粉和酵母膏培养基上菌丝生长较快(日平均生长速率分别为9.63、14.25 mm/d),以山梨醇、乙醇和甘氨酸培养基上较慢(分别为0、0、0.42 mm/d);9种杀菌剂对菌株HZ4111菌丝生长均有较好的抑制作用,其中75%肟菌·戊唑醇水分散粒剂、325 g/L苯甲·嘧菌酯悬浮剂、250 g/L嘧菌酯悬浮剂效果最好,EC50分别为0.000 1、0.000 9、0.000 9μg/m L;70%丙森锌可湿性粉剂、75%百菌清可湿性粉剂、12.5%四氟醚唑水乳剂、325 g/L苯甲·嘧菌酯悬浮剂、75%肟菌·戊唑醇水分散粒剂对病菌孢子萌发有较好的抑制作用,MIC值均小于0.05μg/m L。
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| 关 键 词： | 稻黑孢菌 生物学特性 药剂筛选 |
Biological Characteristics and Fungicides Screening of Nigrospora oryzae |
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| LI Xuqing,FU Hongfei,LI Hongbin.Biological Characteristics and Fungicides Screening of Nigrospora oryzae[J].Journal of Changjiang Vegetables,2016(6). |
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| Authors: | LI Xuqing FU Hongfei LI Hongbin |
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| Abstract: | We studied the biological characteristics of Nigrospora oryzae, and conducted the fungicides screening test in lab. The results showed that, the optimum condition for mycelia growth of HZ4111 was 30℃, 12 h light/12 h dark alternate illumination with pH value of 5.0. Mycelia grew faster on soluble starch medium and yeast extract medium, with average growth rates of 9.63 and 14.25 mm/d respectively, while mycelia grew slower on sorbitol medium, ethanol medium and glycine medium, with average growth rates of 0, 0 and 0.42 mm/d respectively. The nine fungicides had better effect on inhibiting mycelial growth of HZ4111, among them, 75%trifloxystrobin+tebuconazole water dispersible granule (WDG), 325 g/L difenoconazole+azoxystrobin suspension concentrate (SC) and 250 g/L azoxystrobin SC were the most effective inhibitors, with EC50 value of 0.000 1, 0.000 9 and 0.000 9 μg/mL respectively. 70% propineb wettable powder (WP), 75% chlorothalonil WP, 12.5% tetraconazole emulsion in water (EW), 325 g/L difenoconazole+azoxystrobin SC and 75%trifloxystrobin+tebuconazole WDG had better effects on inhibiting spore germination of HZ4111, with MIC value less than 0.05μg/mL. |
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| Keywords: | Nigrospora oryzae Biological characteristics Fungicides screening |
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