| 基于SNP遗传图谱定位甘蓝型油菜分枝角度QTL |
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| 引用本文： | 汪文祥,储文,梅德圣,成洪涛,朱琳琳,付丽,胡琼,刘佳.基于SNP遗传图谱定位甘蓝型油菜分枝角度QTL[J].作物学报,2019,45(1):37-45. |
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| 作者姓名： | 汪文祥 储文 梅德圣 成洪涛 朱琳琳 付丽 胡琼 刘佳 |
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| 作者单位： | 中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室;南漳县植保站 |
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| 基金项目： | This study was supported by the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(Group No. 118);the China Agriculture Research System(CARS-12);the Hubei Agricultural Science and Technology Innovation Center, and the National Natural Science Foundation of China(31471535);the Hubei Agricultural Science and Technology Innovation Center, and the National Natural Science Foundation of China(31771842) |
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| 摘 要： | 分枝角度是油菜株型的重要性状,与油菜的耐密植性密切相关。本研究利用油菜分枝角差异显著的育种亲本材料1098B (分枝角小)和R~2 (分枝角大)杂交获得F1,通过小孢子培养获得含163份株系的DH群体。以油菜60K SNP芯片进行DH群体基因分型,构建高密度遗传图谱,并利用QTL Cartographer 2.5对2个环境下油菜顶端分枝角和基部分枝角进行QTL分析。结果表明,构建的高密度遗传图谱覆盖甘蓝型油菜19条染色体,包含9521个多态性SNP标记, 1442个簇(bin),覆盖基因组长度为2544.07 cM,相邻簇(bin)之间平均距离为1.76 cM。在此图谱基础上采用复合区间作图法(CIM),在2个环境下检测到17个分枝角度QTL,分别位于A01、A02、A03、A06、A09、C02、C03、C04、C06和C08染色体上,单个QTL解释的表型变异为6.39%～21.78%。用比较基因组方法与拟南芥分枝角度同源基因区间比对,鉴定出其中6个QTL的12个候选基因。其中位于A03连锁群QTL在2年的试验中被重复检测到,根据物理位置和基因组信息推测VAMP714为分枝角度的候选基因。这些QTL和候选基因将为油菜分枝角度的遗传改良提供有用的信息。
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| 收稿时间： | 2018-03-22 |
Quantitative trait loci mapping for branch angle and candidate gene screening in Brassica napus L. |
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| Wen-Xiang WANG,Wen CHU,De-Sheng MEI,Hong-Tao CHENG,Lin-Lin ZHU,Li FU,Qiong HU,Jia LIU.Quantitative trait loci mapping for branch angle and candidate gene screening in Brassica napus L.[J].Acta Agronomica Sinica,2019,45(1):37-45. |
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| Authors: | Wen-Xiang WANG Wen CHU De-Sheng MEI Hong-Tao CHENG Lin-Lin ZHU Li FU Qiong HU Jia LIU |
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| Institution: | 1. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China;2. Plant Protection Station of Nanzhang, Xiangyang 441500, Hubei, China |
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| Abstract: | Branch angle is an important agronomic trait of plant architecture. In this study, 163 lines of a DH population derived from a cross between 1019B (compact type) and R2 (loose type) were genotyped by using 60K SNP array and a high-density genetic linkage map was constructed with 1442 bins inclusive of 9521 SNP markers to detect quantitative trait loic (QTL) for basal branch angle and top branch angle. The genetic map contained 19 lingkage groups with a total length of 2544.07 cM and an average distance between adjacent bin-markers of 1.76 cM. Totally, 17 QTL for branch angle were detected on chromosomes A01, A02, A03, A06, A09, C02, C03, C04, C06, and C08, respectively. The phenotypic variation accounted by a single locus was from 6.36% to 21.78%. Twelve candidate genes of branch angle were found underlying six QTL by comparing with homologous genes in Arabidopsis. Candidate gene VAMP714 was close to the peak position of A03 QTL confidence interval, which was identified on chromosome A03 in both environments. These QTL and candidate genes provide useful information for the genetic modification of rapeseed branch angle. |
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| Keywords: | oilseed rape branch angle 60K SNP array QTL mapping candidate gene |
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