Optimization of Antioxidant Peptides Production from the Mantle of Cuttlefish (Sepia pharaonis) Using RSM and Fractionation |
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| Authors: | Ali Hamzeh Saber Khodabandeh Ali Motamedzadegan Mehrdad Noruzinia Joe Mac Regenstein |
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| Institution: | 1. Department of Seafood Processing, Tarbiat Modares University, Noor, Iran;2. Department of Marine Biology, Tarbiat Modares University, Noor, Iran;3. Department of Food Science, Agricultural Sciences and Natural Resources University of Sari, Sari, Iran;4. Department of Hematology, Tarbiat Modares University, Tehran, Iran;5. Department of Food Science, Cornell University, Ithaca, NY, USA |
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| Abstract: | Persian Gulf cuttlefish mantles were hydrolyzed (CPH) using alcalase, and the optimal hydrolysis parameters were obtained for the highest degree of hydrolysis (DH) and strongest antioxidant (based on their ability to quench 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals) activity using response surface methodology (RSM). The predicted optimal parameters of DH and quenching DPPH radicals was: pH of 7.88, 50.2°C, 150 min, and enzyme to substrate ratio of 1.5%. The reducing power (RP) and ability of optimized peptides to quench ABTS radicals in a gastro-intestinal track model system increased during the intestinal stage, while scavenging ability against DPPH radicals dropped (P < 0.05). The oxidation of lipid was retarded in a lecithin-liposome model added with optimized CPH in a concentration dependent response. Ultrafiltration of optimized CPH showed that the 3–10 KDa fraction had the greatest DPPH radical scavenging activity, the 10–30 KDa fraction had the highest reducing power, and the <3 KDa fraction had the greatest ABTS radical scavenging activity. |
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| Keywords: | Antioxidative peptides cuttlefish Sepia pharaonis |
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