| 光温处理对小豆苗期生理性状及叶绿素合成前体的影响 |
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| 引用本文： | 何宁,王雪扬,曹良子,曹大为,洛育,姜连子,孟英,冷春旭,唐晓东,李一丹,万书明,卢环,程须珍.光温处理对小豆苗期生理性状及叶绿素合成前体的影响[J].作物学报,2019,45(3):460-468. |
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| 作者姓名： | 何宁 王雪扬 曹良子 曹大为 洛育 姜连子 孟英 冷春旭 唐晓东 李一丹 万书明 卢环 程须珍 |
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| 作者单位： | 黑龙江省农业科学院;东北农业大学食品学院;中国农业科学院作物科学研究所 |
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| 基金项目： | This study was supported by the Program of Introducing International Super Agricultural Science and Technology (948 Program)(2015-Z54);the China Agriculture Research System(CARS-08-G8) |
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| 摘 要： | 探讨不同低温和光照条件下小豆苗期的冷害发生机制及引起初生叶黄化和叶绿素合成的受阻位点,旨在为小豆耐寒新品种选育及栽培提供理论依据。选择2个对温度和光照反应不同的日本小豆品种,利用人工气候室再现小豆苗期的低温障碍,研究低温遮光处理(昼夜10～13°C, 2%遮光) 18 d和28 d对小豆苗期H_2O_2、SOD、CAT、APX、叶绿素的影响;利用植物生长箱再现小豆苗期的黄化障碍,研究不同的低温处理长度(1 d、3 d、5 d、7 d;10°C, 50μmol m~(–2) s~(–1))和暗处理长度(25°C,黑暗1 d、3 d、5 d、7 d)对绿化后(24 h、25°C、62.5μmol m~(–2) s~(–1))叶绿素合成能力及受阻位点的影响。苗期小豆耐低温和不耐低温品种的最大差异是长期低温遮光处理的H_2O_2含量和SOD活性。长期低温遮光处理后不耐低温品种的H_2O_2含量是耐低温品种的约66倍,但随着绿化处理时间的延长,抗氧化酶活性和叶绿素含量急剧下降直至8 h后消失。与低温处理相比,暗处理才是造成叶绿素合成能力差异的主要原因。对叶绿素合成中间产物的研究表明,从ALA向Proto Ⅸ的转化可能受阻,最终导致叶绿素合成受阻,叶绿素含量下降。说明H_2O_2含量和SOD活性可能与小豆苗期耐冷性关系更密切。引起小豆苗期叶绿素合成受阻位点是Proto Ⅸ的转化。
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| 收稿时间： | 2018-01-04 |
Effects of photoperiods and temperatures on physiological characteristics and chlorophyll synthesis precursors of adzuki bean seedlings |
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| Ning HE,Xue-Yang WANG,Liang-Zi CAO,Da-Wei CAO,Yu LUO,Lian-Zi JIANG,Ying MENG,Chun-Xu LENG,Xiao-Dong TANG,Yi-Dan LI,Shu-Ming WAN,Huan LU,Xu-Zhen CHENG.Effects of photoperiods and temperatures on physiological characteristics and chlorophyll synthesis precursors of adzuki bean seedlings[J].Acta Agronomica Sinica,2019,45(3):460-468. |
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| Authors: | Ning HE Xue-Yang WANG Liang-Zi CAO Da-Wei CAO Yu LUO Lian-Zi JIANG Ying MENG Chun-Xu LENG Xiao-Dong TANG Yi-Dan LI Shu-Ming WAN Huan LU Xu-Zhen CHENG |
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| Institution: | 1.Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China;2.Food Science College, Northeast Agricultural University, Harbin 150030, Heilongjiang, China;3.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | The aim of this study was to discuss the mechanism of chilling injury and the blocked site of chlorophyll synthesis in primary leaf, providing theoretical basis for breeding and cultivation of cold-resistant cultivars. Two Japanese adzuki bean varieties with different temperature and light reactions were used to study the effects of short-term (18 d) and long-term (28 d) low temperature shading treatments (10-13°C, 2% shading) on H2O2, SOD, CAT, APX, and Chl of seedlings in the artificial climate chamber, and the effects of the treatment durations of low temperature (1 d, 3 d, 5 d, 7 d, 10°C, 50 μmol m -2 s -1) and dark (25°C, 1 d, 3 d, 5 d, 7 d) under chlorophyll synthesis blocked site (25°C, and under illumiation at 62.5 μmol m -2 s -1for 24 h). The content of H2O2 and activity of SOD had significant difference between cold resistant and susceptible varieties during seedling stage. The content of H2O2 in susceptible variety was 66 folds of the resistant one, and with the greening treatment antioxidant activity and content of chlorophyll were rapidly dropped until totally vanished after 8 h. The main cause of difference in chlorophyll synthesis between the two varieties was the dark treatment but not the low temperature treatment. It was suggested that the transformation from δ-ALA to Proto IX might be blocked in chloroplast stroma, which eventually inhibited chlorophyll synthesis and decreased chlorophyll content. It is suggested that H2O2 content and SOD activity may be more closely related to the cold tolerance of adzuki bean at seedling stage. The transformation of Proto IX is the blocking site in chlorophyll synthesis which causes etiolated seedlings of adzuki bean. |
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| Keywords: | adzuki bean light temperature treatment antioxidant enzymes chlorophyll synthesis |
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