| 山东小麦黄花叶病毒的分子检测与鉴定 |
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| 引用本文： | 丛倩倩,许斐斐,李向东,张广民.山东小麦黄花叶病毒的分子检测与鉴定[J].山东农业科学,2012,44(11). |
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| 作者姓名： | 丛倩倩 许斐斐 李向东 张广民 |
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| 作者单位： | 山东农业大学植物保护学院植物病理学系,山东泰安,271018 |
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| 基金项目： | 国家行业性公益项目"粮食作物重要病毒防控技术研究与示范 |
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| 摘 要： | 利用RT-PCR对2012年山东10个地区送检的105个小麦样品进行了检测,从泰安、临沂、枣庄3个地区的样品中检测到了小麦黄花叶病毒(Wheat yellow mosaic virus,WYMV)。这3个地区WYMV分离物CP基因的核苷酸一致率为97.6%～98.3%,氨基酸一致率为98.3%～99.0%。在利用CP基因核苷酸序列构建的系统进化树中,这3个分离物均与WYMV分离物聚类到一起,其中枣庄分离物和河南分离物(FR828007)亲缘关系更近。
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| 关 键 词： | 小麦黄花叶病毒 CP基因 系统进化分析 |
Molecular Detection and Identification of Wheat Yellow Mosaic Virus in Shandong Province |
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| Cong QianQian
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Xu FeiFei
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Li XiangDong
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Zhang GuangMin.Molecular Detection and Identification of Wheat Yellow Mosaic Virus in Shandong Province[J].Shandong Agricultural Sciences,2012,44(11). |
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| Authors: | Cong QianQian Xu FeiFei Li XiangDong Zhang GuangMin |
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| Abstract: | One hundred and five wheat samples collected from 10 districts of Shandong Province were detected for wheat yellow mosaic virus(WYMV) using RT-PCR.WYMV was detected from samples from Taian,Linyi and Zaozhuang.The coat protein genes of WYMV from these three areas shared the nucleotide identity of 97.6%~98.3% and the amino acid identity of 98.3%~99.0%.In the phylogenetic tree constructed based on the nucleotide sequence,the three isolates were clustered together with other WYMV isolates.The isolates from Zaozhuang had closer genetic relationships with the isolate from Henan. |
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| Keywords: | Wheat yellow mosaic virus CP gene Phylogenetic analysis |
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