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Genetic Identification of a New Small Grain Dwarf Gene in Rice
作者姓名:WuCheng  LIXiu-lan  DENGXiao-jian  WANGPing-rong  LIRen-duan  YANGZhi-rong
作者单位:[1]CollegeofLifeScience,SichuanUniversity,Chengdu610064,China [2]RiceResearchInstitute,SichuanAgriculturalUniversity,Chengdu611130,China
摘    要:The plant material used in the study was rice line 162d, a new small grain dwarf mutant. Polymorphic analysis of 221 SSR loci demonstrated that 162d derived from a semidwarf variety Shuhui 162 through mutation, and 162d and Shuhui 162 were just a pair of near isogenic lines. Genetic analysis of Fl and F2 populations suggested that dwarfism in 162d was controlled by a single recessive gene. Phenotypic characteristics of the mutant gene were that plant height was about a quarter of normal height, grain size about a quarter of normal size, leaf was short and broad, and seed setting rate was very low,compared with the near isogenic line Shuhui 162. The mutant gene was sensitive to gibberellin (GA3) treatment and did not located on the region near the centromere of rice chromosome 5, where dl gene located. Therefore, it was concluded that the mutant gene of 162d was a new small grain dwarf gene in rice.

关 键 词:水稻  SSR标记  基因鉴定  矮杆突变体

Genetic Identification of a New Small Grain Dwarf Gene in Rice
WuCheng LIXiu-lan DENGXiao-jian WANGPing-rong LIRen-duan YANGZhi-rong.Genetic Identification of a New Small Grain Dwarf Gene in Rice[J].Rice science,2003,11(1):11-15.
Authors:WU Cheng  LI Xiu-lan  DENG Xiao-jian  WANG Ping-Rong  LI Ren-duan  YANG Zhi-rong
Abstract:The plant material used in the study was rice line 162d, a new small grain dwarf mutant. Polymorphic analysis of 221 SSR loci demonstrated that 162d derived from a semidwarf variety Shuhui 162 through mutation, and 162d and Shuhui 162 were just a pair of near isogenic lines. Genetic analysis of F1 and F2 populations suggested that dwarfism in 162d was controlled by a single recessive gene. Phenotypic characteristics of the mutant gene were that plant height was about a quarter of normal height, grain size about a quarter of normal size, leaf was short and broad, and seed setting rate was very low,compared with the near isogenic line Shuhui 162. The mutant gene was sensitive to gibberellin (GA3) treatment and did not located on the region near the centromere of rice chromosome 5, where dl gene located. Therefore, it was concluded that the mutant gene of 162d was a new small grain dwarf gene in rice.
Keywords:rice  dwarf gene  genetic identification  genetic marker  gene location  near isogenic line
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