| 甜瓜细菌性软腐病病原鉴定 |
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| 引用本文： | 葛林梅,李红叶,张明方,杜一丰.甜瓜细菌性软腐病病原鉴定[J].果树学报,2006,23(3):427-430. |
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| 作者姓名： | 葛林梅 李红叶 张明方 杜一丰 |
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| 作者单位： | 1. 浙江大学农业与生物技术学院,杭州,310029
2. 义乌市农业局种植业管理总站,浙江,义乌,322000 |
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| 摘 要： | 近年来,浙江义乌等地的甜瓜(CucumismeloL.)果实上普遍发生一种外观褐色小斑,果肉水渍状腐烂现象,局部田块发病率高,损失严重。为确定病原,从病果上分离到4个细菌菌株,分别定名为M1、M2、M3和M4。烟草过敏性反应和致病性测定表明M2和M4不具致病性,而M1和M3在烟草上能引起典型的过敏性坏死反应,接种甜瓜24h内即导致果实的明显腐烂,所引起的腐烂症状与田间发病果实一致。对M1和M3进行培养性状、革兰氏染色、形态学和鞭毛观察、马铃薯薯块腐烂试验,以及16SrRNA基因测序和比较,结果证明该病害是由软腐欧氏杆菌软腐亚种Erwiniacarotovorasubsp.carotovora引起。
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| 关 键 词： | 甜瓜 果实腐烂 病原鉴定 软腐欧氏杆菌软腐亚种 |
| 文章编号： | 1009-9980（2006）03-427-04 |
| 收稿时间： | 2005-10-26 |
| 修稿时间： | 2006-02-17 |
Identification of the pathogen inducing fruit soft rot of melon |
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| GE Lin-mei,LI Hong-ye,ZHANG Ming-fang,DU Yi-feng.Identification of the pathogen inducing fruit soft rot of melon[J].Journal of Fruit Science,2006,23(3):427-430. |
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| Authors: | GE Lin-mei LI Hong-ye ZHANG Ming-fang DU Yi-feng |
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| Institution: | 1 College of Agricuhure and Biotechnology,Zhejiang University,Hangzhou ,Zhejiang 310029 China; 2 Department of Agricuhure ,Yiwu municipality, Yiwu, Zhejiang 322000 China |
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| Abstract: | Soft rot with symptoms of initial brown spots on the surface and soft rot inside of melon (Cucumis melo L.) was often reported in Yiwu and other areas, Zhejiang province. The infected melon fruit corrupted consequently. In some areas this disease caused significant yield loss to melon industry. 4 isolates (M1 to M4) with two types of morphology of bacterial colonies were isolated and purified from infected melon fruit. The tests of hypersensitive reaction in tobacco (Nicotiana tabacum L.) and pathogenicity in the melon fruit for these isolates demonstrated that M1 and M3 were pathogenic, whereas M2 and M4 were not. Both M1 and M3 were identified to be an isolate of Erwinia carotovora subsp. carotovora based on their properties of morphology, flagella, gram reaction, host range and the sequence alignment of 16S rRNA gene. In addition, inoculation test showed that this pathogen could induce soft rot to tomato, cucumber, watermelon and pumpkin fruits. |
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| Keywords: | Melon (Cucumis melo L ) Fruit soft rot Pathogen identification Erwinia carotovora subsp carotovora |
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