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| 铜对体外仔猪软骨细胞增殖和细胞骨架的影响 | |
| 引用本文： | 刘国文,冯海华,张乃生,王哲.铜对体外仔猪软骨细胞增殖和细胞骨架的影响[J].畜牧与兽医,2005,37(6):13-16. |
| 作者姓名： | 刘国文 冯海华 张乃生 王哲 |
| 作者单位： | 1. 解放军军需大学军事兽医系,吉林,长春,130062;内蒙古民族大学动物科技学院,内蒙古,通辽,028042 2. 内蒙古民族大学动物科技学院,内蒙古,通辽,028042 3. 解放军军需大学军事兽医系,吉林,长春,130062 |
| 基金项目： | 国家自然科学基金项目（39970567、30170713）. |
| 摘 要： | 体外分离、培养仔猪关节软骨细胞,在细胞培养液中分别添加铜0、7.8、15.6、31.2、62.5μmol/L。结果表明,软骨细胞在4种铜浓度中可存活并增殖,但随铜浓度的增加,其存活率、增殖率、3H-TdR掺入率有明显的差异,且能破坏软骨细胞骨架。培养液中添加铜31.2μmol/L,对软骨细胞的增殖作用最强,增殖率、3H-TdR掺入数显著高于对照组(P<0.01),软骨细胞形态及骨架均正常。表明31.2μmol/L铜浓度是促进体外软骨细胞增殖的最适浓度。 |
| 关 键 词： | 铜 猪 软骨细胞 增殖 细胞骨架 |
| 文章编号： | 0529-5130（2005）06-0013-04 |
| 收稿时间： | 11 9 2004 12:00AM |
| 修稿时间： | 2004-11-09 |
Effect of copper on proliferation and cellular skeleton in the young pig chondrocytes in vitro |
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| LIU Guo-wen,FENG Hai-hua,ZHANG Nai-sheng,WANG Zhe.Effect of copper on proliferation and cellular skeleton in the young pig chondrocytes in vitro[J].Animal Husbandry & Veterinary Medicine,2005,37(6):13-16. | |
| Authors: | LIU Guo-wen FENG Hai-hua ZHANG Nai-sheng WANG Zhe |
| Abstract: | Chondrocytes from the lateral trochler ridge of the distal femur in one-day-old pig were cultured in medium supplemented with 0, 7.8, 15.6, 31.2, 62.5 μmol/L copper. DNA synthesis in chondrocytes was measured by ~3H-thymindine incorporation. The results showed that proliferation-promoting activity and incorporation of ~3H-thymindine in chondrocytes increased in culture medium added with 7.8,15.6,31.2 and 62.5 μmol/L Cu. The optimal copper concentration for the proliferation-promoting activity of chondrocytes was 31.2 μmol/L. |
| Keywords: | copper pigs chondrocyte proliferation cellular skeleton |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |