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太子参叶斑病抗性诱导因子筛选
引用本文:林丛发,翁琳琳,钟爱清,陈芦根.太子参叶斑病抗性诱导因子筛选[J].中国生物防治,2011,27(3):383-387.
作者姓名:林丛发  翁琳琳  钟爱清  陈芦根
作者单位:福建省宁德市农业科学研究所,福安,355017
基金项目:福建省宁德市科技局资助项目(20090053)
摘    要:为筛选诱导太子参产生对叶斑病抗性的诱导因子,在离体培养条件下,用水杨酸、草酸、壳聚糖、磷酸氢二钾、亚硒酸钠和太子参叶斑病粗毒素液这6种因子诱导处理太子参组培苗,在1~7d内测定叶片相关酶活性的变化。诱导培养7d后,用太子参叶斑病粗毒素液对组培苗进行离体叶片针刺接种抗性鉴定,结果表明,诱导处理后能明显增强太子参叶片内POD、PPO和PAL的活性;6种因子均能诱导太子参对叶斑病产生抗性,以190mg.L-1和380mg.L-1草酸的诱抗效果较好,分别达到76.1%和74.7%,极显著高于其它诱导因子的诱抗效果。

关 键 词:诱导抗性  太子参  诱导因子  叶斑病菌

Screening of Activators Inducing Resistance on Leaf Spot Disease (Phyllosticta commonsii) in Taizishen(Pseudostellaria heterophylla)
LIN Cong-fa,WENG Lin-lin,ZHONG Ai-qing,CHEN Lu-gen.Screening of Activators Inducing Resistance on Leaf Spot Disease (Phyllosticta commonsii) in Taizishen(Pseudostellaria heterophylla)[J].Chinese Journal of Biological Control,2011,27(3):383-387.
Authors:LIN Cong-fa  WENG Lin-lin  ZHONG Ai-qing  CHEN Lu-gen
Institution:LIN Cong-fa,WENG Lin-lin,ZHONG Ai-qing,CHEN Lu-gen(Ning De Agriculture institute,Fu'an 355017,China)
Abstract:To screen activators on leaf spot disease(Phyllosticta commonsii) of Taizishen(Pseudostellaria heterophylla),in vitro-regenerated Taizishen plantlets were treated under aseptic conditions with 6 kinds of inducing factors including salicylic acid,oxalic acid,chitosan,potassium hydrogen phosphate,sodium selenite and crude Phyllosticta commonsii toxin.The activity of related enzymes in leaves 1-7 days after treatment was determined.Disease reaction of the treated plantlets was identified by pin-prick inoculati...
Keywords:inducing resistance  Taizishen  activators  Phyllosticta commonsii  
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