| 西瓜枯萎菌拮抗细菌的筛选、鉴定及防效测定 |
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| 引用本文： | 岳菊,刘邮洲,张荣胜,刘永锋,王治林,陈志谊.西瓜枯萎菌拮抗细菌的筛选、鉴定及防效测定[J].中国生物防治,2011,27(3):428-432. |
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| 作者姓名： | 岳菊 刘邮洲 张荣胜 刘永锋 王治林 陈志谊 |
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| 作者单位： | 1. 南京农业大学植物保护学院植物病理学系,南京210095;江苏省农业科学院植物保护研究所,南农210014
2. 江苏省农业科学院植物保护研究所,南农,210014 |
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| 基金项目： | 江苏省农业科技自主创新资金项目(CX09615,CX112022) |
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| 摘 要： | 从内蒙古、新疆、安徽、河南、江苏、江西6省的西瓜、黄瓜和茄子3种作物根际土壤和病健组织中,共获得样本64份,分离得到细菌1050株。室内抑菌活性测定结果表明：9株细菌分离物对西瓜枯萎病菌Fusariumoxysporumf.sp.niveum、甘蓝黑斑病菌Alternaria brassicicola、辣椒疫霉病菌Phytophthora capsici和番茄灰霉病菌Botrytis cinerea均具有很强的拮抗作用,其中菌株NBT-15和PL-82对西瓜枯萎病菌菌丝生长的抑制作用比较明显,抑制率分别为60.5%和55.3%。盆栽防病试验结果表明：NBT-15和PL-82对西瓜枯萎病的防治效果分别为80.4%和75.6%。形态特征、生理生化特性及16S rDNA序列分析结果确定菌株NBT-15为枯草芽孢杆菌Bacillus subtilis,PL-82为铜绿假单胞菌Pseudomonas aeruginosa。
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| 关 键 词： | 拮抗细菌 西瓜枯萎病菌 防效 鉴定 |
Screening and Identification of Antagonistic Bacteria Strains against Fusarium oxysporum f.sp.niveum |
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| YUE Ju,LIU You-zhou,ZHANG Rong-sheng,LIU Yong-feng,WANG Zhi-lin,CHEN Zhi-yi.Screening and Identification of Antagonistic Bacteria Strains against Fusarium oxysporum f.sp.niveum[J].Chinese Journal of Biological Control,2011,27(3):428-432. |
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| Authors: | YUE Ju LIU You-zhou ZHANG Rong-sheng LIU Yong-feng WANG Zhi-lin CHEN Zhi-yi |
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| Institution: | YUE Ju1,2,LIU You-zhou2,ZHANG Rong-sheng1,LIU Yong-feng2,WANG Zhi-lin2,CHEN Zhi-yi2(1.College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,2.Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China) |
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| Abstract: | In this paper,1050 bacterial strains were isolated from the rhizosphere soil and roots of watermelon,cucumber,and eggplant in Inner Mongolia,Xinjiang,Anhui,Henan,Jiangsu and Jiangxi province.Among the tested strains,9 strains could inhibit mycelia growth of Fusarium oxysporum f.sp.niveum,Alternaria brassicicola,Phytophthora capsici and Botrytis cinerea.Two strains,NBT-15 and PL-82,had strong inhibitory activity against F.oxysporum,with inhibition rate of 60.5% and 55.3%,respectively.In pot experiment,contro... |
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| Keywords: | antagonistic bacteria Fusarium oxysporum f sp niveum control efficiency identification |
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