| AA肉种鸡内源性类ALV-J gp85基因及其抗原表位的分析 |
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| 引用本文： | 郭文龙,朱瑞良,崔治中,闫振贵,马荣德,谭燕玲,朱明华,王新建.AA肉种鸡内源性类ALV-J gp85基因及其抗原表位的分析[J].中国兽医学报,2011,31(4). |
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| 作者姓名： | 郭文龙 朱瑞良 崔治中 闫振贵 马荣德 谭燕玲 朱明华 王新建 |
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| 作者单位： | 山东农业大学动物科技学院,山东泰安,271018 |
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| 基金项目： | 山东省农业重大应用技术创新课题; 农业部公益性行业科研专项(200803019) |
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| 摘 要： | 利用ALV-J gp85基因两侧的序列片段为引物,从山东潍坊某肉种鸡场送检的7日龄肉种鸡基因组中完整地扩增了内源性类ALV-J gp85基因。利用ALV-J env基因特异性引物不能扩增出长度为2.2kb的目的片段,用ALV-J特异性单抗JE-9做间接免疫荧光检测,也呈现阴性反应。这种内源性类ALV-J gp85基因与已报道的禽内源性反录病毒EAV-HP序列的同源性为85.6%～93.8%,其中与ADOL 0系来航鸡EAV-HP的相似性最高(93.8%)。所得到的内源性类ALV-J gp85基因与外源性ALV-J毒株HPRS-103、SD07LK1、NX0101的同源性分别为93.8%、91.8%和91.7%。并且与外源性ALV-J gp85基因具有相似的Jameson-Worlf抗原表位优势。这种内源性类gp85基因很可能与肉雏鸡早期感染ALV-J后所呈现的免疫耐受现象有关。
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| 关 键 词： | 内源性反录病毒 内源性类ALV-J gp85基因 聚合酶链式反应 |
Analysis of avian endogenous ALV-J gp85-like gene and its epitope in AA meat-type broiler breeder |
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| GUO Wen-long,ZHU Rui-liang,CUI Zhi-zhong,YAN Zhen-gui,MA Rong-de,TAN Yan-ling,ZHU Ming-hua,WANG Xin-jian.Analysis of avian endogenous ALV-J gp85-like gene and its epitope in AA meat-type broiler breeder[J].Chinese Journal of Veterinary Science,2011,31(4). |
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| Authors: | GUO Wen-long ZHU Rui-liang CUI Zhi-zhong YAN Zhen-gui MA Rong-de TAN Yan-ling ZHU Ming-hua WANG Xin-jian |
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| Institution: | GUO Wen-long,ZHU Rui-liang*,CUI Zhi-zhong,YAN Zhen-gui,MA Rong-de,TAN Yan-ling,ZHU Ming-hua,WANG Xin-jian |
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| Abstract: | primers corresponding to the nucleotide sequences flanking the ALV-J gp85 gene were used to amplify the intact endogenous gp85-like gene from 7-day-old AA meat-type broiler breeder collected in Weifang region,Shandong province.The isolate strain was negative reaction in polymerase chain reaction with specific primers of ALV-J env gene and gave no reaction in indirect fluorescence antibody assay(IFA)with ALV-J specific monoclonal antibody JE-9.The endogenous ALV-J gp85-like gene had 85.6% to 93.8% homology w... |
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| Keywords: | avian endogenous retrovirus endogenous ALV-J gp85-like gene polymerase chain reaction |
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