| 牛结核分枝杆菌重组多抗原表位基因的原核表达与鉴定 |
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| 引用本文： | 冯向辉,包永占,王建永,赵月兰,左玉柱,秦建华.牛结核分枝杆菌重组多抗原表位基因的原核表达与鉴定[J].中国兽医学报,2011,31(1). |
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| 作者姓名： | 冯向辉 包永占 王建永 赵月兰 左玉柱 秦建华 |
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| 作者单位： | 1. 河北农业大学,动物科技学院,河北,保定071000
2. 河北农业大学,中兽医学院,河北,定州,073000 |
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| 基金项目： | 国家“十一五”科技支撑计划资助项目(2006BAD04A10-4); 河北省重大技术创新专项计划资助项目(07227146Z-4) |
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| 摘 要： | 利用DNAStar对牛结核分枝杆菌的3个主要抗原mpb70、mpb83、esat-6进行预测分析,将预测的抗原指数高的抗原表位经全基因合成后,克隆到表达载体pET28a(+)中,构建重组质粒pET28a-mpb-70-83-esat-6,转化入大肠杆菌BL21(DE3)中诱导表达,表达产物经SDS-PAGE鉴定,结果检测到相对分子质量约为21 000的重组蛋白。经NI-NTA纯化重组蛋白后进行Western-blot鉴定,结果显示该重组蛋白可被牛结核分枝杆菌的多克隆抗体识别,表明该蛋白具有良好的反应原性。
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| 关 键 词： | 牛结核分枝杆菌 mpb70 mpb83 esat-6 原核表达 |
Expression of recombinant epitope genes of Mycobacterium tuberculosis and identification of its expression product |
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| FENG Xiang-hui,BAO Yong-zhan,WANG Jian-yong,ZHAO Yue-lan,ZUO Yu-zhu,QIN Jian-hua.Expression of recombinant epitope genes of Mycobacterium tuberculosis and identification of its expression product[J].Chinese Journal of Veterinary Science,2011,31(1). |
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| Authors: | FENG Xiang-hui BAO Yong-zhan WANG Jian-yong ZHAO Yue-lan ZUO Yu-zhu QIN Jian-hua |
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| Institution: | FENG Xiang-hui1,BAO Yong-zhan1,WANG Jian-yong2,ZHAO Yue-lan2,ZUO Yu-zhu1,QIN Jian-hua1(1.College of Animal Science and Technology,Hebei Agricultural University,Baoding,Hebei 071000,China,2.College of Chinese Traditional Veterinary Science,Dingzhou,Hebei 073000,China) |
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| Abstract: | The three major antigens of Mycobacterium tuberculosis,mpb70,mpb83 and esat-6 were analyzed by DNAStar.The epitope which is in a high antigenic index was synthesised and cloned into the pET-28a(+) expression vector.Constructed a recombinant plasmid called pET28a-mpb-70-83-esat-6.The recombinant plasmid was transformed into E.coli BL21 competent cells and then induced by 1 mmol/L IPTG.SDS-PAGE analysis revealed that a band of approximately 21 000 in molecular weight was expressed from the induced E.coli BL21... |
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| Keywords: | Mycobacterium tuberculosis mpb70 mpb83 esat-6 prokaryotic expression |
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