| Mx基因抗性位点在11个不同鸡种中的分布及遗传结构分析 |
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| 引用本文： | 栾德琴,常国斌,包文斌,盛中伟,陈国宏.Mx基因抗性位点在11个不同鸡种中的分布及遗传结构分析[J].中国兽医学报,2011,31(1). |
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| 作者姓名： | 栾德琴 常国斌 包文斌 盛中伟 陈国宏 |
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| 作者单位： | 扬州大学动物科学与技术学院,江苏,扬州,225009 |
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| 基金项目： | 国家“十一五”863重点项目(2008AA101009); 国家“十一五”科技支撑计划重点项目(2008BADB2D07) |
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| 摘 要： | 通过错配PCR技术检测Mx基因S631N位点的抗性等位基因A和敏感性等位基因G在11个不同鸡种中的分布差异。结果显示,错配PCR-RFLP能准确检测出抗性等位基因A与敏感性等位基因G在不同鸡种内的突变,抗性等位基因A在所有群体内的基因频率平均为0.502,敏感性等位基因G的频率平均为0.498;11个不同鸡种群体Mx基因S631N位点的观察杂合度平均为0.506 7,Shannon信息指数平均为0.562 9。在该位点上,11个鸡种,漳州斗鸡、吐鲁番斗鸡、皖南鸡、文昌鸡、如皋鸡、安卡鸡偏离了Hardy-Weinberg平衡(P<0.05)外,其余5个群体均处Hardy-Weinberg平衡状态(P>0.05);经Ewens-Watterson中立性检验,该位点在各群体内(除如皋鸡群体外)均属于中立性选择。基于该位点等位基因频率构建的UPGMA聚类图将11个不同鸡种分为3大类,聚类结果反映了11个不同鸡种在Mx基因抗性方面存在的差异和优势。
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| 关 键 词： | 鸡种 Mx基因 遗传结构 聚类分析 |
Genetic distribution and population genetic structure analysis of antiviral resistant Mx gene locus in 11 chicken breeds |
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| LUAN De-qin,CHANG Guo-bin,BAO Wen-bin,SHENG Zhong-wei,CHEN Guo-hong.Genetic distribution and population genetic structure analysis of antiviral resistant Mx gene locus in 11 chicken breeds[J].Chinese Journal of Veterinary Science,2011,31(1). |
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| Authors: | LUAN De-qin CHANG Guo-bin BAO Wen-bin SHENG Zhong-wei CHEN Guo-hong |
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| Institution: | LUAN De-qin,CHANG Guo-bin,BAO Wen-bin,SHENG Zhong-wei,CHEN Guo-hong(College of Animal Science and Technology,Yangzhou University,Yangzhou,Jiangsu 225009,China) |
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| Abstract: | In the experiment,the mismatch PCR-RFLP technique was used to detect the differences of the resistant and sensitive alleles distribution of S631N locus of Mx gene in eleven chicken populations.The results showed that the PCR-RFLP technique properly detected the mutation between resistant allele A and sensitive allele G.Among the eleven populations,the average frequencies of resistant allele A and sensitive allele G were 0.502 and 0.498,respectively.The average value of observed heterozygosis and Shannon's i... |
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| Keywords: | chicken breed Mx gene genetic structure clustering analysis |
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