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| 用于土壤中生防芽孢杆菌B006和BH1检测的SCAR标记及其特异性 | |
| 引用本文： | 耿文义,郭荣君,李世东,许修宏.用于土壤中生防芽孢杆菌B006和BH1检测的SCAR标记及其特异性[J].中国生物防治,2011,27(2):233-240. |
| 作者姓名： | 耿文义 郭荣君 李世东 许修宏 |
| 作者单位： | 1. 中国农业科学院植物保护研究所/农业部生物防治重点开放实验室,北京,100081;东北农业大学农业资源与环境学院,哈尔滨,150030 2. 中国农业科学院植物保护研究所/农业部生物防治重点开放实验室,北京,100081 3. 东北农业大学农业资源与环境学院,哈尔滨,150030 |
| 基金项目： | 国家大宗蔬菜产业技术体系建设项目(nycytx-35); 中央级公益性科研院所基本科研业务费专项资金资助项目(2010YW04) |
| 摘 要： | 生防芽孢杆菌Bacillus spp.菌株B006和BH1对引发多种根腐病的真菌具有拮抗作用,研发特异性标记是研究该菌株在土壤中生态行为的关键.本研究采用16个RAPD随机引物对B006和BH1以及包括芽孢杆菌、鞘氨醇杆菌Sphingosinc spp.和假单胞菌Pseudomonas spp.在内的25株参试菌株的基囚组DNA多态性进行了分析,其中利用单引物B19和C01可分别扩增到仅出现于B006和BH1基因图谱中的特异性条带(大小分别为730bp和658bp).为提高扩增的特异性和稳定性,根据B006和BH1的RAPD标记中核苷酸序列分别设计了6对和12对引物并用于菌株特异性条带的筛选,从中挑选出2对特异性最好的引物转化为SCAR引物,命名为SCAR-B006和SCAR-BH1,扩增产物大小分别为523bp和658bp.在自然土中进一步验证SCAR标记的特异性,结果表明仅在加有菌株B006或BH1的土壤样品中可扩增到相应的SCAR条带,说明了这种以PCR为基础的方法可用于土壤中菌株B006和BH1的快速鉴别. |
| 关 键 词： | SCAR分子标记 RAPD分子标记 芽孢杆菌 生物防治 |
Identification of the SCAR Markers for Detection of Biocontrol Bacillus Strains B006 and BH1 in Soil |
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| GENG Wen-yi , GUO Rong-jun , LI Shi-dong , XU Xiu-hong.Identification of the SCAR Markers for Detection of Biocontrol Bacillus Strains B006 and BH1 in Soil[J].Chinese Journal of Biological Control,2011,27(2):233-240. | |
| Authors: | GENG Wen-yi GUO Rong-jun LI Shi-dong XU Xiu-hong |
| Institution: | GENG Wen-yi1,2,GUO Rong-jun1,LI Shi-dong1,XU Xiu-hong2(1.Key Laboratory for Biological Control of Ministry of Agriculture,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100081,2. School of Resources and Environment,Northeast Agricultural University,Harbin 150030,China) |
| Abstract: | Two Bacillus strains,B006 and BH1,are highly suppressive against Fusarium spp.,the causal agents of plant root diseases.Discrimination of these strains from native soil bacteria was critical to study their population dynamics and behaviors in soil.Polymorphism in genomic DNA of the strains B006 and BH1 as well as 25 references including Bacillus spp.,Sphingosine spp.and Pseudomonas spp.were analyzed with 16 random primer pairs by RAPD.Sequence-specific fragments with 730 bp and 658 bp could be obtained from... |
| Keywords: | SCAR RAPD Bacillus spp biological control |
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