| 杨树叶枯病菌rDNA ITS序列验证及生物学特性研究 |
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| 引用本文： | 计红芳,杨谦,宋瑞清.杨树叶枯病菌rDNA ITS序列验证及生物学特性研究[J].林业研究,2006,17(1):17-20. |
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| 作者姓名： | 计红芳 杨谦 宋瑞清 |
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| 作者单位： | 哈尔滨工业大学，哈尔滨工业大学，东北林业大学 哈尔滨150010，哈尔滨150010，哈尔滨150040黑龙江科学院资源研究所，哈尔滨150040 |
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| 基金项目： | ThispaperwassupportedbyNationalNaturalScienceFoundationofChina(No.30271083). |
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| 摘 要： | 首次应用通用引物ITS1与ITS4从杨树叶枯病菌中扩增出目的片段为601bp的rDNAITS序列(登录号为DQ011257)。与GenBank核酸序列数据库进行比对表明:本菌株与Alternariaalternata(AY787684)及Alternariaalternate(AY354228)相似率为98%,验证为Alternariaalternate。杨树叶枯病菌生物学特性如下:分生孢子萌发最适温度25℃,最适pH为6。菌丝生长最适温度为25℃,在10℃与30℃时生长极其缓慢,高于35℃时,生长停止;菌丝生长最适pH为6,在PDA 杨树煎汁培养基上生长最好。
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| 关 键 词： | 杨树叶枯病菌 Alternaria alternata rDNA ITS序列 生物学特性 |
| 收稿时间： | 15 August 2005 |
| 修稿时间： | 25 November 2005 |
Biological characters and rDNA ITS sequences of pathogen of poplar leaf blight |
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| JI Hong-fang,YANG Qian,SONG Rui-qing.Biological characters and rDNA ITS sequences of pathogen of poplar leaf blight[J].Journal of Forestry Research,2006,17(1):17-20. |
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| Authors: | JI Hong-fang YANG Qian SONG Rui-qing |
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| Institution: | (1) Harbin Institute of Technology, Harbin, 150001, P. R. China;(2) Northeast Forestry University, Harbin, 150040, P. R. China;(3) Research Institute of Natural Resources, Heilongjiang Academy of Sciences, Harbin, 150040, P. R. China |
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| Abstract: | DNA was extracted from the strain of pathogen of poplar leaf blight using a modified CTAB method. ITS sequence (601bp) was initially amplified from the pathogen by using the universal primers ITS1 and ITS4 (registered No. DQ011257). Comparing to the nucleo-tide sequences acquired from GenBank database, the strain is clustered into the homogeneity with Alternaria alternate (AY787684) and Al-ternaria alternate (AY354228), with a homology of 98%, thus the strain was checked as Alternaria alternata (Fr.) Keissler. The optimal conditions for conidia germination and mycelium growth of the pathogen were tested. The optimal temperature for conidia germinating and mycelium growth is 25oC, and the optimal pH value is 6. Mycelium grows rather slowly at 10 oC and 30 oC and growth stops at above 35 oC. Among the six culture mediums tested, PDA poplar leaf juice medium is most favorable for mycelium growth. |
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| Keywords: | Pathogen Popular leaf blight Alternaria alternate rDNA ITS sequences Biological characteristics |
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