| 抗猪瘟IgY的制备及临床应用初报 |
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| 引用本文： | 张振仓,沈文正,许信刚,张国祥.抗猪瘟IgY的制备及临床应用初报[J].西北农业学报,2003,12(3):36-38. |
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| 作者姓名： | 张振仓 沈文正 许信刚 张国祥 |
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| 作者单位： | 1. 杨陵职业技术学院,陕西,杨凌,712100
2. 西北农林科技大学,陕西,杨凌,712100 |
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| 基金项目： | 杨凌职业技术学院科研基金(B52002002). |
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| 摘 要： | 应用合理的免疫程序,用猪瘟抗原免疫蛋鸡,并以水稀释法、聚乙二醇萃取法结合透析对所产蛋的卵黄部分进行处理,成功地提取到抗猪瘟IgY(SF-IgY),获得率为6.83mg/mL。该制剂纯度为45.14％,能中和1000MID猪瘟兔化种毒,用于紧急治疗猪瘟后,临床应用效果确实。
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| 关 键 词： | 猪瘟 IgY提取 中和试验 临床应用 |
| 文章编号： | 1004-1389(2003)03-0036-03 |
| 收稿时间： | 2002/3/15 0:00:00 |
| 修稿时间： | 2002年3月15日 |
Preliminary Study on Preparation and Clinical Application of IgY against Swine Fever |
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| ZHANG Zhen-cang,SHEN Wen-zheng,XU Xin-gang and ZHANG Guo-xiang.Preliminary Study on Preparation and Clinical Application of IgY against Swine Fever[J].Acta Agriculturae Boreali-occidentalis Sinica,2003,12(3):36-38. |
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| Authors: | ZHANG Zhen-cang SHEN Wen-zheng XU Xin-gang and ZHANG Guo-xiang |
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| Institution: | Yangling Vocational and Technical College, Yangling Shaanxi 712100, China;Yangling Vocational and Technical College, Yangling Shaanxi 712100, China;Northwest Sci-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China;Northwest Sci-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China |
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| Abstract: | Layer hens were immunized with swine fever antigen under proper immunity schedule. The egg yolk was collected and tested after the treatments with water dilution, PGE extraction and dialysis. Then the IgY preparation against swine fever (SF-IgY)was successfully produced, with the purification degree of 45. 14% and production rate of 6. 83 mg/mL. The SF-IgY product could neutralize 1000MID Swine Fever virus hatched with rabbit and showed reliable effect in urgent clinical treatment against Swine Fever. |
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| Keywords: | Swine fever preparation of IgY Neutralization test Clinical application |
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