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| 新疆沙冬青抗冻蛋白基因保守区序列的克隆及其分析 | |
| 引用本文： | 詹彬,安沙舟,杨丽娟,李莲瑞,马春晖.新疆沙冬青抗冻蛋白基因保守区序列的克隆及其分析[J].新疆农业科学,2009,46(6):1307-1310. |
| 作者姓名： | 詹彬 安沙舟 杨丽娟 李莲瑞 马春晖 |
| 作者单位： | 1. 新疆农业大学草业与环境科学学院,乌鲁木齐,830052;塔里木大学动物科学学院/新疆兵团塔里木畜牧科技重点实验室,新疆阿拉尔,843300 2. 新疆农业大学草业与环境科学学院,乌鲁木齐,830052 3. 塔里木大学动物科学学院/新疆兵团塔里木畜牧科技重点实验室,新疆阿拉尔,843300 |
| 基金项目： | 国家教育部科学技术研究重点项目 |
| 摘 要： | 【目的】从Genbank数据库中选择与新疆沙冬青(Ammopiptanthus nanus)亲缘关系最近的同属蒙古沙冬青(Ammopiptanthus mongolicus)抗冻蛋白(AFP)基因AY590122、AY843521、AY843522的mRNA序列,设计扩增新疆沙冬青AFP基因保守区引物。【方法】以新疆沙冬青为研究材料,提取其总RNA,利用RT-PCR技术克隆出新疆沙冬青AFP基因的保守区域。【结果】序列分析表明,新疆沙冬青与蒙古沙冬青的保守区域同源性达98.15%。【结论】得到新疆沙冬青的抗冻蛋白的保守区域。 |
| 关 键 词： | 新疆沙冬青 抗冻蛋白 克隆 序列分析 |
Cloning and Sequence Analysis of AFP Gene Conservative Region in Ammopiptanthus Nanus |
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| ZHAN Bin,AN Sha-Zhou,YANG Li-Juan,LI Lian-Rui,MA Chun-Hui.Cloning and Sequence Analysis of AFP Gene Conservative Region in Ammopiptanthus Nanus[J].Xinjiang Agricultural Sciences,2009,46(6):1307-1310. | |
| Authors: | ZHAN Bin AN Sha-Zhou YANG Li-Juan LI Lian-Rui MA Chun-Hui |
| Abstract: | Objective]A pair of primers were designed according to the three antifreezing proteins (AFP) gene of Ammopiptanthus mongolicus (Genbank accession number is AY590122, AY843521, and AY843522, respectively). Method]Total RNA of Ammopiptanthus nanus was extracted using Trizol, which then was used as a template to amplify conservative region of AFP gene by RT-PCR in Ammopiptanthus nanus.Result]The result showed that the conservative region of AFP gene of Ammopiptanthus nanus had high homology and similarity (98.15%) to the AFP gene of Ammopiptanthus mongolicus.Conclusion]The above result could also set the base for the complete AFP gene of Ammopiptanthus nanus |
| Keywords: | Ammopiptanthus nanus AFP clone sequence analysis |
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