| 苹果抗寒矮化砧木71-3-150茎尖培养快繁体系建立 |
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| 引用本文： | 王淼淼,马晓月,张学英,徐继忠.苹果抗寒矮化砧木71-3-150茎尖培养快繁体系建立[J].云南林业科技,2014(6):44-47. |
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| 作者姓名： | 王淼淼 马晓月 张学英 徐继忠 |
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| 作者单位： | 河北农业大学园艺学院,河北保定071001 |
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| 基金项目： | 河北省科技厅资助项目（14226307D-5）及农业部公益性行业科研专项资助（201203075-05）. |
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| 摘 要： | 以苹果抗寒矮化砧木71-3-150的茎尖为外植体，通过研究培养基中不同植物生长调节剂配比对芽苗诱导、增殖和生根的影响，筛选出适合71-3-150砧木茎尖组培快繁的培养基配方。结果表明，以MS为基本培养基，附加蔗糖30 g／L、琼脂6 g／L，适宜启动培养的植物生长调节剂为6-BA 1.0 mg／L＋NAA 0.05 mg／L；适宜增殖培养的植物生长调节剂为6-BA 1.0 mg／L＋NAA 0.05 mg／L，增殖系数可达到每4周4.75倍。以1／2MS （蔗糖15 g／L，琼脂6 g／L）为基本培养基，适宜组培苗生根的植物生长调节剂为IAA 1.0 mg／L＋IBA 0.6 mg／L，生根率达100％，每株苗平均生根9.6条，移栽成活率达90％以上。
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| 关 键 词： | 苹果砧木71—3-150 茎尖培养 组培快繁 植物生长调节剂 |
Study on Rapid Micropropagation Technique of Shoot Tip Culture of Cold-Resistant Apple Dwarf Rootstock 71-3-150 |
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| WANG Miao-miao,MA Xiao-yue,ZHANG Xue-ying,XU Ji-zhong.Study on Rapid Micropropagation Technique of Shoot Tip Culture of Cold-Resistant Apple Dwarf Rootstock 71-3-150[J].Yunnan Forestry Science and Technology,2014(6):44-47. |
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| Authors: | WANG Miao-miao MA Xiao-yue ZHANG Xue-ying XU Ji-zhong |
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| Institution: | (College of Horticulture, Agricultural University of Hebei, Baoding Hebei 071001, P. R. China) |
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| Abstract: | Taking the shoot tips of the cold-resistant apple dwarf rootstock 71-3-150 as experimental materials, effects of different plant growth regulators on tissue-cultured seedling induction, multiplication and rooting were re-searched.The optimal medium formula was selected.The results showed that the basic medium was MS supplemen-ted with sugar 30 g/L and agar 6 g/L, and the optimal plant growth regulators of start culture medium that suited the multiplication were 6-BA 1.0 mg/L＋NAA 0.05 mg/L.The optimal plant growth regulators of subculture medium were 6-BA 1.0 mg/L＋NAA 0.05 mg/L, and the coefficient of multiplication were exceeded 4.75 times every four weeks.For the 1/2 MS supplemented with sugar 15 g/L and agar 6 g/L as basic medium, optimal plant growth reg-ulators of rooting were IAA 1.0 mg/L＋IBA 0.6 mg/L, the rooting rate of shoots was 100%, and the average num-ber of rooting was 9.6.Then the rooted plantlets were transplanted, and the survival rate was more than 90%. |
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| Keywords: | apple rootstock 71-3-150 shoot tip culture rapid micropropagation plant growth regulators |
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