| 草地螟中肠运载蛋白LstiSLC25基因的克隆及表达 |
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| 引用本文： | 尹姣,郭巍,李克斌,宇文秋杰,曹雅忠.草地螟中肠运载蛋白LstiSLC25基因的克隆及表达[J].农业生物技术学报,2009,17(3):516-521. |
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| 作者姓名： | 尹姣 郭巍 李克斌 宇文秋杰 曹雅忠 |
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| 作者单位： | 中国农业科学院植物保护研究所 |
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| 摘 要： | 利用甜菜夜蛾Spodoptera exigua（Hübner）围食膜多克隆抗体免疫筛选草地螟（Loxostege sticticalis L．）中肠cDNA表达文库，得到编码运载蛋白LstiSLC25的全长cDNA克隆。该cDNA克隆全长1399bp(GenBank登录号EU924506)，开放阅读框长897 bp，编码299个氨基酸，预测分子量和等电点分别为32.1kD和9.46。序列含有三个典型的溶质运转重复结构，具有溶质运载蛋白家族的典型特征。将该基因与pET30载体重组后，经IPTG诱导，蛋白在大肠杆菌中获得了表达。
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| 关 键 词： | 草地螟，cDNA表达文库，LstiSLC25，原核表达 |
| 收稿时间： | 2008-9-9 |
| 修稿时间： | 2009-2-26 |
Cloning and expression of LstiSLC25 gene from Loxostege sticticalis L. midgut |
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| Abstract: | Using the polyclonal antiserum against Spodoptera exigua (Hübner) invertebrate intestinal mucin (IIM), a solute carrier gene LstiSLC25 was screened from the cDNA expression library of Loxostege sticticalis midgut. The full-length of LstiSLC25 is 1399bp (GenBank accession no. EU924506) and the open reading frame(ORF) was 897bp, encoding 299 amino acid residues; the predicted MW and pI were 32.1kD and 9.46, respectively. The LstiSLC25 possesses three solcars, which is characterized of solute carrier family. The LstiSLC25 gene has been recombined into pET30 vector. The Western blot analysis demonstrated that LstiSLC25 protein was expressed at low level after being induced by IPTG. |
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