Effect of simulated transport stress on the rat small intestine: A morphological and gene expression study |
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| Authors: | Changrong Wan Peng Yin Xiaolong Xu Mingjiang Liu Shasha He Shixiu Song Fenghua Liu Jianqin Xu |
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| Institution: | 1. CAU-BUA TCVM Teaching and Researching Team, College of Veterinary Medicine, China Agricultural University (CAU), Beijing 100193, PR China;2. College of Animal Science and Technology, Beijing University of Agriculture (BUA), Beijing 102206, PR China |
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| Abstract: | The present study investigated the effects of simulated transport stress on morphology and gene expression in the small intestine of laboratory rats. Sprague Dawley rats were subjected to 35 °C and 0.1×g on a constant temperature shaker for physiological, biochemical, morphological and microarray analysis before and after treatment. The treatment induced obvious stress responses with significant decreases in body weight (P < 0.01), increases in rectal temperature, serum corticosterone (CORT), serum glucose (GLU), creatine kinase (CK) and lactate dehydrogenase (LDH) levels (P < 0.01), as well as expression of Hsp27/70/90 mRNA (P < 0.05; P < 0.01). The rat jejunum was severely damaged and apoptotic after mimicking transport stress, which may mainly be related to cell death, oxidation reduction and hormone imbalance determined by microarray analysis. The bioinformatics analysis from the present study would provide insight into the potential mechanisms underlying transport stress-induced injury in the rat small intestine. |
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| Keywords: | Transport stress Intestinal damage Microarray analysis Morphological study |
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