Effect of different methods for the induction of spermiation on semen quality in European eel |
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| Authors: | Juan F Asturiano Luz Pérez Daniel L Garzón David S Peñaranda Francisco Marco-Jiménez Silvia Martínez-Llorens Ana Tomás & Miguel Jover |
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| Institution: | Grupo de Investigación en Recursos Acuícolas, Departamento de Ciencia Animal, Universidad Politécnica de Valencia, Camino de Vera, Valencia, Spain; Centro de Investigación y Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias, Ctra. Náquera-Moncada, Moncada, Valencia, Spain |
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| Abstract: | Five hormonal treatments with human chorionic gonadotropin (hCG) were tested for the induction of maturation and spermiation in male farmed eels. The main aim was to optimize previously used hormonal treatments to achieve shorter induction treatments, longer spermiation periods and/or higher sperm quality. Fish treated for just 3 weeks (treatment E) or until the onset of spermiation (treatment C) showed the worst results, while the treatment consisting of weekly administration of 1.5 IU hCG g?1 fish (treatment A) induced the highest percentage of spermiating males, the highest number of sperm samples and sperm volumes and densities similar to the rest of the treatments (B: half hormone dosage, or D: biweekly administration). Evaluation of the sperm quality was performed by computer‐assisted sperm analysis (CASA), considering the percentage of total motile spermatozoa, the percentage of fast and medium‐velocity spermatozoa, as well as different motility parameters. Sperm samples from A‐D groups showed between 44% and 54% motile spermatozoa, and between 10% and 15% fast spermatozoa, while samples from E‐treated males showed 0% motile cells. No significant differences were found in the spermatozoa straight line velocity (VSL), curvilinear velocity (VCL) or the angular velocity (VAP), neither spermatozoa beating cross frequency (BCF) between A–D groups. |
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| Keywords: | Anguilla anguilla European eel sperm activation media CASA |
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