| 以诺丽茎段为外植体的无籽基因转化研究 |
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| 引用本文： | 贾丹丹,蓝增全,吴田.以诺丽茎段为外植体的无籽基因转化研究[J].西南农业大学学报,2018,40(9):7-12. |
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| 作者姓名： | 贾丹丹 蓝增全 吴田 |
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| 作者单位： | 西南林业大学园林学院/国家林业局风景园林工程技术研究中心;西南绿色发展研究院 |
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| 基金项目： | 云南省应用基础研究计划项目（2016FB049）；国家林业局风景园林重点学科经费（501313）；西南林业大学2017年大学生科技创新项目（C17071）. |
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| 摘 要： | 以诺丽无菌苗不带腋芽的茎段为转化材料,采用根癌农杆菌介导法,将含有无籽基因的质粒pCAMBIA1305.1导入到诺丽细胞中,通过Kan快速筛选、PCR检测等方法对转化子进行鉴定.实验结果表明:抗性芽的平均分化频率为42%,经过PCR检测,以茎为外植体进行遗传转化的转化率为5.4%.在转化过程中,有些植株可能会发生饰变.该实验初步证实已把无籽基因转入诺丽,后续需Southern杂交进一步验证.
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| 关 键 词： | 海巴戟 茎段 农杆菌 转基因 |
| 收稿时间： | 2017/12/7 0:00:00 |
Studies on Genetic Transformation of Seedlessness Gene to Noni (Morinda citrifolia) with Its Stem Segments as the Explants |
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| JIA Dan-dan,LAN Zeng-quan,WU Tian.Studies on Genetic Transformation of Seedlessness Gene to Noni (Morinda citrifolia) with Its Stem Segments as the Explants[J].Journal of Southwest Agricultural University,2018,40(9):7-12. |
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| Authors: | JIA Dan-dan LAN Zeng-quan WU Tian |
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| Institution: | 1. Garden College, Southwest Forestry University/Southwest Landscape Architecture Engineering Research Center of State Forestry Administration, Kunming 650224, China;2. Southwest Green Development Institute, Kunming 650224, China |
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| Abstract: | In this study, the method of Agrobacterium-mediation was used to import pCAMBIA 1305.1, which carries the seedlessness gene, into the cells of the stem segments without axillary buds of aseptic noni (Morinda citrifolia). Then we identified the transformant with the methods of Kan quick screening and PCR checking. PCR results showed that the average differentiation rate of resistant buds was 42%, and the conversion rate of the genetic transformation using noni stem segments as the explants was 5.4%. Furthermore, in the process of transformation, modification could occur in some plants. This study preliminarily confirmed that the seedlessness gene had been transformed into the noni plants, but Southern hybridization is to be made in further study to verify the results of this study. |
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| Keywords: | Morinda citrifolia stem segment Arobacterium genetic transformation |
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