| 鸡源致病性大肠杆菌的分离鉴定及耐药性分析 |
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| 引用本文： | 周瑶琴,游凤,高彤,付雅慧,王豪举,丁红雷.鸡源致病性大肠杆菌的分离鉴定及耐药性分析[J].西南农业大学学报,2018,40(5):23-29. |
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| 作者姓名： | 周瑶琴 游凤 高彤 付雅慧 王豪举 丁红雷 |
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| 作者单位： | 西南大学兽医传染病学实验室 |
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| 基金项目： | 中央高校基本科研业务费专项资金资助项目(XDJK2017D034)；国家重点基础研究发展计划资助项目(2013CB12720)；重庆市社会民生科技创新专项资助项目(cstc2015shmszx80033) |
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| 摘 要： | 分离鉴定某蛋鸡养殖场病鸡的病原并对病原的部分重要生物学特性进行研究.通过病理剖检、细菌和菌落形态观察、分子生物学鉴定、生化试验和动物回归试验,确定从8只病鸡分离的21株病原菌为大肠杆菌.对30种抗菌药物的药物敏感性试验分析表明,这些菌株存在广泛的多重耐药,特别是对青霉素G、阿莫西林、卡那霉素、甲氧嘧啶、复方新诺明、四环素、氯霉素和万古霉素耐药严重,对头孢哌酮、头孢曲松、头孢他啶、氨曲南、阿米卡星、妥布霉素较为敏感.对超广谱β-内酰胺酶(ESBL)表型及基因型分析可知,所有菌株均为产ESBL大肠杆菌,其主要的ESBL耐药基因为bla_(TEM).
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| 关 键 词： | 大肠杆菌 分离鉴定 PCR 药物敏感性试验 超广谱β-内酰胺酶 |
Isolation, Identification and Antimicrobial Resistance Analysis of Chicken-Associated Pathogenic Escherichia coli |
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| ZHOU Yao-qin,YOU Feng,GAO Tong,FU Ya-hui,WANG Hao-ju,DING Hong-lei.Isolation, Identification and Antimicrobial Resistance Analysis of Chicken-Associated Pathogenic Escherichia coli[J].Journal of Southwest Agricultural University,2018,40(5):23-29. |
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| Authors: | ZHOU Yao-qin YOU Feng GAO Tong FU Ya-hui WANG Hao-ju DING Hong-lei |
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| Abstract: | In order to provide reference for integrated disease prevention and control of hen farms, a research was made to isolate and identify pathogens from a layer farm in Chongqing. Pathological examination, bacterial and clonal morphological examination, molecular biological identification, biochemical tests and animal regression test of 21 strains isolated from eight sick chickens on the farm showed that they were all Escherichia coli strains. Susceptibility test of 30 antimicrobials showed that all the isolates were multi-drug resistant. They were highly resistant to penicillin G, amoxicillin, kanamycin, sulfameter, trimethoprim-sulfamethoxazole, tetracycline, chloramphenicol and vancomycin, and fairly sensitive to cefoperazone, ceftriaxone, ceftazidime, aztreonam, amikacin and tobramycin. The phenotype and genotype analyses of the extended spectrum beta-lactamases (ESBLs) showed that the isolates were all ESBLs-producing E. coli and the main ESBL resistant gene was blaTEM. |
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