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| 胰岛素、胰高血糖素对体外培养脂肪细胞胰高血糖素受体mRNA丰度的影响 | |
| 引用本文： | 张永宏,孙玉成,高妍,宋文华,刘国文,王雪莹,李小兵,王哲.胰岛素、胰高血糖素对体外培养脂肪细胞胰高血糖素受体mRNA丰度的影响[J].中国兽医学报,2007,27(6):862-864,886. |
| 作者姓名： | 张永宏 孙玉成 高妍 宋文华 刘国文 王雪莹 李小兵 王哲 |
| 作者单位： | 1. 吉林大学,畜牧兽医学院,吉林,长春,130062 2. 吉林大学,实验动物中心,吉林,长春,130062 3. 吉林农业大学,动物科技学院,吉林,长春,130118 |
| 摘 要： | 为阐明胰岛素、胰高血糖素在奶牛脂肪代谢中的调控作用,应用RT-PCR法观察了胰岛素、胰高血糖素对体外培养脂肪细胞胰高血糖素受体(GLNR)mRNA丰度的影响。结果发现,随着培养液中胰岛素质量浓度的升高,GLNR mRNA表达逐渐增加(P<0.05);而随着培养液中胰高血糖素浓度的升高,GLNR mRNA表达逐渐降低(P<0.01)。本研究结果表明,胰岛素、胰高血糖素直接调控奶牛脂肪细胞胰高血糖素受体mRNA的表达。 |
| 关 键 词： | 胰岛素 胰高血糖素 脂肪细胞 胰高血糖受体mRNA |
| 文章编号： | 1005-4545（2007）06-0862-03 |
| 修稿时间： | 2006-05-19 |
Effects of glucagon and insulin on expression of GLNR mRNA by cultured neonatal calf lipocytes in vitro |
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| ZHANG Yong-hong,SUN Yu-cheng,GAO Yan,SONG Wen-hua,LIU Guo-wen,WANG Xue-ying,LI Xiao-bing,WANG Zhe.Effects of glucagon and insulin on expression of GLNR mRNA by cultured neonatal calf lipocytes in vitro[J].Chinese Journal of Veterinary Science,2007,27(6):862-864,886. | |
| Authors: | ZHANG Yong-hong SUN Yu-cheng GAO Yan SONG Wen-hua LIU Guo-wen WANG Xue-ying LI Xiao-bing WANG Zhe |
| Institution: | 1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China; 2. The Center of Laboratory Animal, Jilin University, Changchun 130062, China; 3. College of Animal Science and Veterinary Medicine, Jilin Agricultural University, Changchun 130118, China |
| Abstract: | In order to investigate the regulation roles of glucagon and insulin in the lipometabolism of dairy cows,the effects of bovine insulin and glucagon were abserved on expression of glucagon receptor(GLNR)mRNA of cultured neonatal calf lipocytes by RT-PCR.The expression of GLNR mRNA increased(P<0.05) with increasing concentrations of insulin in the media;and when glucagon in the media increased gradually,the expression of GLNR mRNA decreased(P<0.01).These results indicate insulin and glucagon could regulate the expressions of cultured hepatocellular GLNR mRNA directly. |
| Keywords: | insulin glucagon lipocyte GLNR mRNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |