Ploidy level studies on the <Emphasis Type="Italic">Dioscorea cayenensis</Emphasis>/<Emphasis Type="Italic">Dioscorea rotundata</Emphasis> complex core set |
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| Authors: | J Obidiegwu J Loureiro E Ene-Obong E Rodriguez M Kolesnikova-Allen C Santos C Muoneke R Asiedu |
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| Institution: | 1.International Institute of Tropical Agriculture, c/o L. W. Lambourn & Co.,Croydon,UK;2.Department of Agronomy,Michael Okpara University of Agriculture,Umudike,Nigeria;3.National Root Crops Research Institute,Umuahia,Nigeria;4.CESAM & Department of Biology,University of Aveiro,Aveiro,Portugal;5.Biotechnology Unit Tun Abdul Razak Research Centre Brickendonbury,Hertford,UK;6.Department of Botany, Faculty of Science,Charles University in Prague,Prague 2,Czech Republic |
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| Abstract: | The Guinea yams, Dioscorea
cayenensis Lam. and D. rotundata Poir. (D. cayenensis–D. rotundata complex), represent a highly important crop, widely distributed in the humid and semi-humid tropics. The ploidy levels of
170 accessions of the core set of Guinea yams from West African countries was determined using flow cytometry with propidium
iodide staining. One hundred and eight of the genotypes were found to be tetraploid, 47 were hexaploid and five were octoploid.
One mixoploid individual containing tetraploid and hexaploid nuclei was also detected. A deeper analysis considering each
separate taxon revealed that while for D. rotundata the majority of individuals were tetraploid, for D. cayenensis this ploidy level was not detected in any of the accessions. Also, no association between ploidy level and place of cultivation
was found for the evaluated germplasm. The obtained data is highly valuable for breeding programs of Guinea yam, especially
for the optimization of future hybridization experiments directed to the genetic improvement of this economically important
crop. |
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| Keywords: | Breeding Dioscorea cayenensis/D rotundata Flow cytometry Genetic resources Germplasm Ploidy level |
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