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| 空肠弯曲杆菌耐药性及其主要相关基因初步分析 | |
| 引用本文： | 姜毅,杨凤娥,侯建军,朱建国,华修国,杨志彪.空肠弯曲杆菌耐药性及其主要相关基因初步分析[J].中国兽药杂志,2008,42(12). |
| 作者姓名： | 姜毅 杨凤娥 侯建军 朱建国 华修国 杨志彪 |
| 作者单位： | 1. 上海交通大学农业与生物学院,上海,200240 2. 宁夏灵武市动物疾病预防控制中心,宁夏灵武,751400 3. 上海交通大学农业与生物学院,上海200240;内蒙古农业大学动物科学与医学学院,呼和浩特010018 |
| 基金项目： | 上海市标准化项目 |
| 摘 要： | 从307份鸡盲肠内容物样品中分离到47株空肠弯曲杆菌,并测定这些空肠弯曲杆菌耐药性,结果显示对喹诺酮类药(环丙沙星)耐药性为31.9%;对氨苄青霉素,克林霉素和红霉素耐药性较高,分别达到85.1%、83.0%和83.0%;而对痢特灵和庆大霉素则较为敏感,敏感度分别为85.1%和74.5%。有89.4%的菌株显示多重耐药性。利用MAMAPCR技术,对获得的47株空肠弯曲杆菌进行检测,结果显示对环丙沙星耐药的15株空肠弯曲杆菌均检测出其在gyrA基因257位发生点突变,30株对喹诺酮药物敏感的菌株均未检出。另外2株对环丙沙星耐药的菌株,有1株检测出点突变。 |
| 关 键 词： | 空肠弯曲杆菌 耐药性 相关基因 |
Analysis of Antimicrobial Resistance of Campylobacter jejuni and Its Related Genes |
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| JIANG Yi,YANG Fen-ge,HOU Jian-jun,ZHU Jian-guo,HUA Xiu-guo,YANG Zhi-biao.Analysis of Antimicrobial Resistance of Campylobacter jejuni and Its Related Genes[J].Chinese Journal of Veterinary Drug,2008,42(12). | |
| Authors: | JIANG Yi YANG Fen-ge HOU Jian-jun ZHU Jian-guo HUA Xiu-guo YANG Zhi-biao |
| Institution: | JIANG Yi1,YANG Feng-e3,HOU Jian-jun1,2,ZHU Jian-guo1,HUA Xiu-guo1,YANG Zhi-biao1 |
| Abstract: | 47 Strains of C.jejuni were isolated from 307 fowl appendix contents,and antimicrobial resistance of these bacterium was tested by disk diffusion technique.The result indicated that 31.9% of strains demonstrated resistance to ciprofloxacin,the high level of resistance seen were 85.1% to ampicillin,83.0% to clindamycin,83.0% to erythromycin,and C.jejuni was more sensitive to furaxone(85.1%)and gentamicin(74.5%).89.4% strains demonstrated multiple drug resistance.Mismatch amplification mutation assay PCR was used to detect the mutation of gyrA.The result indicated that 15 strains of C.jejuni which showed resistance to ciprofloxacin had mutation at ribonucleotide position 257 in the gyrA sequence of C.jejuni isolated,while other 30 sensitive ones had no mutation.Additionally,mutation was detected in one of the two strains of C.jejuni which were resistance to ciprofloxacin,and the mutation was confirmed by sequencing. |
| Keywords: | Campylobacter jejuni antimicrobial resistance related genes |
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