| 多花黄精组织培养快繁技术的研究 |
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| 引用本文： | 刘红美,方小波,夏开德,杨苏文.多花黄精组织培养快繁技术的研究[J].种子,2010,29(12). |
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| 作者姓名： | 刘红美 方小波 夏开德 杨苏文 |
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| 作者单位： | 贵阳医学院医学生物技术教研室; |
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| 基金项目： | 贵州省高层次人才科研条件特助经费项目，贵阳医学院青年基金 |
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| 摘 要： | 目的]:建立多花黄精组织培养快速繁殖体系。方法]:以多花黄精带芽根茎为外植体,消毒后将其置于富含不同激素配比的培养基中培养,筛选各阶段合适的培养基。结果]:在附加有2,4-D的诱导培养基上出现不定芽,增殖培养以MS+6-BA4.0 mg/L+2,4-D0.2 mg/L为好,增殖倍数可达10倍。在增殖培养基中加入GA3有利于壮苗。6-BA、2,4-D、GA3组合更加有利于形成粗壮无根苗。培养基1/2 MS+IBA0.7 mg/L最适合用于诱导黄精不定芽生根,生根率可达95%。结论]:2,4-D比NAA更有利于多花黄精的不定芽诱导。该繁殖体系可在短时间内提供大量黄精种苗。
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| 关 键 词： | 多花黄精 组织培养 快繁体系 |
Study on Rapid Micropropagation Technology by Tissue Culture of P. cyrtonemaHua. |
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| LIU Hong-mei,FANG Xiao-bo,XIA Kai-de,YANG Su-wen.Study on Rapid Micropropagation Technology by Tissue Culture of P. cyrtonemaHua.[J].Seed,2010,29(12). |
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| Authors: | LIU Hong-mei FANG Xiao-bo XIA Kai-de YANG Su-wen |
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| Institution: | LIU Hong-mei,FANG Xiao-bo,XIA Kai-de,YANG Su-wen(Department of Medical Biotechnology,Guiyang Medical College,Guiyang 550004,China) |
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| Abstract: | Objective]:To develop a rapid micropropagation system by tissue culture for P.cyrtonemaHua.Methods]:The rhizomes that had sprouted of P.cyrtonemaHua as explants were cultured on MS medium supplemented with different concentrations of BA in combination with different concentrations of NAA or 2,4-D to select optimized medium for different phase.Results]:Multiple shoots were only got in medium supplemented with 2,4-D.The solid MS medium with 6-BA(2.0mg/L) and 2,4D(0.1mg/L) was suitable for the multiplacatio... |
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| Keywords: | P cyrtonemaHua tissue culture micropropagation system |
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