| 花生种子脂肪氧化酶的活性测定研究 |
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| 引用本文： | 侯美玲,苗华荣,陈静,王绍斌.花生种子脂肪氧化酶的活性测定研究[J].安徽农业科学,2008,36(32). |
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| 作者姓名： | 侯美玲 苗华荣 陈静 王绍斌 |
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| 作者单位： | 沈阳农业大学农学院,辽宁沈阳,110161;山东省花生研究所,山东青岛,266100 |
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| 基金项目： | 山东省农业科学院青年基金
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山东省农业良种工程项目
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| 摘 要： | 目的]为了研究花生种子脂肪氧化酶的适宜测定条件。方法]采用紫外分光光度法测定花生种子脂肪氧化酶的活性。结果]结果表明:花生种子脂肪氧化酶最适反应体系为浓度0.10 mol/L pH值5.8的磷酸缓冲液和浓度0.20 mol/L pH值8.0的磷酸缓冲液;最适反应温度为35℃;最适酶液添加量为30μl;最适测定时间为1 min。此外,酶液保存在4℃条件下,脂肪氧化酶的活性随时间的延长而逐渐下降。结论]该研究为进一步探讨花生种子脂肪氧化酶的生理功能奠定了基础。
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| 关 键 词： | 花生 脂肪氧化酶 酶活 |
Measurement of Peanut Seeds Lipoxygenase Activity |
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| Abstract: | Objective] The research aimed to study the suitable measurement condition for peanut lipoxygenase activities.Method] Spectrophotometric assay was used to measure the activity of peanut lipoxygenase.Result] The results showed that the optimal reaction systems were concentration 0.10 mol/L pH value 5.8 and concentration 0.20 mol/L pH value 8.0 phosphate buffer solution,the optimal temperature was 35 ℃,the optimal added content of peanut lipoxygenase solution was 30 μl,the optimal measure time was 1 minute.More over,when it was conserved at 4 ℃,the activity of peanut lipoxygenase was fallen with time.Conclusion] This study layed a good foundation for research on the physiological function of peanut lipoxygenase activity. |
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| Keywords: | Peanut Lipoxygenase Activity |
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