| 绿盲蝽危害后转Bt基因棉花嫩叶的几个生理指标变化特征分析 |
| |
| 引用本文： | 刘昱,张培通,陈兵林,郭文琦,张恒友.绿盲蝽危害后转Bt基因棉花嫩叶的几个生理指标变化特征分析[J].棉花学报,2013,25(1):51-56. |
| |
| 作者姓名： | 刘昱 张培通 陈兵林 郭文琦 张恒友 |
| |
| 作者单位： | 1. 南京农业大学农学院,南京210095;农业部长江下游棉花与油菜重点实验室,江苏省农业科学院经济作物研究所,南京210014
2. 农业部长江下游棉花与油菜重点实验室,江苏省农业科学院经济作物研究所,南京210014 |
| |
| 基金项目： | 江苏省自然科学基金项目，江苏省农业科技自主创新资金项目(CX |
| |
| 摘 要： | 以转Bt基因棉品系苏抗310和其轮回亲本泗棉3号为材料，研究绿盲蝽危害后转Bt基因棉花顶部嫩叶的几个相关生理指标的变化特征。结果表明：绿盲蝽危害后，棉花顶叶的叶绿素、可溶性蛋白和游离氨基酸含量显著降低，说明绿盲蝽危害后，棉花顶部嫩叶氮代谢受到一定破坏，引起叶绿素含量的下降，这对叶片成熟后光合作用是不利的。棉花顶部嫩叶受危害后，缩合单宁含量提高和苯丙氨酸解氨酶（PLA）活性增强，表明棉花植株抵御盲蝽危害能力提高，但转Bt基因棉品系顶部嫩叶缩合单宁含量和PLA活性低于非转Bt基因棉品种；即使是绿盲蝽危害后的顶部嫩叶也均未达到非转Bt基因棉品种正常水平，这可能是导致转Bt基因棉盲蝽危害加重的原因之一。棉花顶部嫩叶受害后，非转Bt基因棉泗棉3号可溶性糖含量降低，而转Bt基因棉品系苏抗310升高；非转Bt基因棉品种受绿盲蝽危害后顶部嫩叶可溶性糖含量降低，不利于绿盲蝽的发生，而转Bt基因棉品系受危害后顶部嫩叶可溶性糖含量上升，不利于对绿盲蝽的危害控制，这可能是转Bt基因棉绿盲蝽危害重于非转Bt基因棉的又一原因。
|
| 关 键 词： | 转Bt基因棉 常规棉 绿盲蝽 生理指标 |
| 收稿时间： | 2012-05-09; |
Analysis on the Changes of Physiological Indices of Young Leaves Infected by Lygus lucorum of Transgenic Bt Cotton |
| |
| LIU Yu
,
ZHANG Pei-tong
,
CHEN Bing-lin
,
GUO Wen-qi
,
ZHANG Heng-you.Analysis on the Changes of Physiological Indices of Young Leaves Infected by Lygus lucorum of Transgenic Bt Cotton[J].Cotton Science,2013,25(1):51-56. |
| |
| Authors: | LIU Yu ZHANG Pei-tong CHEN Bing-lin GUO Wen-qi ZHANG Heng-you |
| |
| Institution: | 1. College of Agricultural, Nanjing Agricultural University, Nanjing 210095, China; 2. Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture, China, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China |
| |
| Abstract: | The experiment was carried out in 2011, at the Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences. The Bt transgenic cotton cultivars(Sukang 310) and its recurrent parent(Simian 3) were used as the experimental materials to investigate the changes on physiological indices of the young leaves after the cotton were infected by Lygus lucorum. Infected by Lygus lucorum, the free amino acids contents, the soluble protein contents and the chlorophy contents of the young leaves of both the transgenic Bt cotton and non-transgenic Bt cotton were decreased significantly, and the condensed tannin contents and phenylalanine ammonia-lyase(PLA) activities of young leaves were increased significantly, in both bud stage and boll stage. Infected by Lygus lucorum, the soluble sugar content of young leaves of transgenic Bt cotton was increased, while that of non-transgenic Bt cotton was decreased. It's indicated that after damaged by Lygus lucorum, the nitrogenous metabolism of young leaves was decreased, and led to the chlorophyll content decrease. It's harmful to the photosynthesis of the leaves grown mature. Increasing in the condensed tannin contents and PLA activities was advantageous to withstanding infecting of Lygus lucorum. While the condensed tannin contents and PLA activities of the young leaves damaged by Lygus lucorum of the transgenic Bt cotton was lower than that of the normal young leaves of non-transgenic Bt cotton. It was one of the possible cause of the change on the population dynamics and damage of Lygus lucorum. Increasing in the soluble sugar content was advantageous to the infecting of Lygus lucorum. While damaged by Lygus lucorum, the increasing in soluble sugar content of young leaves of transgenic Bt cotton was advantageous to infecting of Lygus lucorum. It was another possible cause of the change on the population dynamics and damage of Lygus lucorum. |
| |
| Keywords: | Bt cotton non-transgenic Bt cotton Lygus lucorum Lygus lucorum physiological index |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《棉花学报》浏览原始摘要信息 |
| 点击此处可从《棉花学报》下载免费的PDF全文 |
|
