Effect of exogenous ovine placental lactogen on basal and prostaglandin-stimulated progesterone production by porcine luteal cells |
| |
| Authors: | Gregoraszczuk E L Gertler A Futoma E |
| |
| Institution: | Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Ingardena 6, 30-060 Cracow, Poland. greg@zuk.iz.uj.edu.pl |
| |
| Abstract: | The ability of ovine placental lactogen (oPL) to stimulate progesterone secretion of porcine luteal cells isolated from ovaries in different stages of the oestrous cycle and to support the luteotropic action of PGE2 or to protect the corpus luteum (CL) against the luteolytic action of PGF2 alpha was investigated. oPL in all doses used had no effect on progesterone production of cells isolated from early developing corpora lutea while in doses of 1 and 10 ng/ml it increased oestradiol secretion by this type of cells. In doses of 1, 10 and 100 ng/ml it also increased progesterone secretion of cells isolated from mature corpora lutea in a dose-dependent manner. No influence on progesterone production of cells isolated from regressing corpora lutea was observed. oPL added to the culture media had no effect on PGE2-stimulated progesterone production by cells isolated from mature corpora lutea. However, it exerted a protective effect against the luteolytic action of PGF2 alpha observed in cultures treated with PGF2 alpha alone or in combination with PGE2 in a ratio of 4:1. These studies provide evidence that oPL is luteotropic and supports progesterone production in swine. The fact that oPL acted directly on ovarian steroidogenesis suggests that it may also play some role under non-pregnant physiological conditions. Future studies of structural and functional proteins secreted by the porcine conceptus will help resolve this uncertainty. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
