| 抑制性消减杂交技术在对虾基因克隆中的应用 |
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| 引用本文： | 赵永贞,蒋伟明,陈秀荔,李咏梅,张彦明,黎铭,陈晓汉.抑制性消减杂交技术在对虾基因克隆中的应用[J].动物医学进展,2008,29(4):76-80. |
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| 作者姓名： | 赵永贞 蒋伟明 陈秀荔 李咏梅 张彦明 黎铭 陈晓汉 |
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| 作者单位： | 1. 广西水产研究所广西水产遗传育种与健康养殖重点实验室,广西南宁,530021
2. 西北农林科技大学动物医学院,陕西杨陵,712100 |
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| 基金项目： | 广西科学研究与技术开发计划项目 |
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| 摘 要： | 抑制性消减杂交技术是一项筛选、分离未知差异表达基因的试验方法,该技术具有快速、简便、灵敏、特异、高效,所需起始样本量较少等优点。利用该技术构建对虾组织消减cDNA文库,鉴定差异表达相关基因及其表达特性。通过对对虾差异性表达的免疫相关基因、抗病基因及其他性状基因的了解,旨在为进一步研究对虾抗病机理奠定基础,为利用抗病基因进行抗病育种提供一个有效途径,为提高对虾的经济效益提供前景。
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| 关 键 词： | 抑制性消减杂交 对虾 差异表达基因 cDNA文库 |
| 文章编号： | 1007-5038(2008)04-0076-05 |
| 修稿时间： | 2008年1月2日 |
Application of Suppression Subtractive Hybridization in Shrimp Gene Cloning |
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| ZHAO Yong-zhen,JIANG Wei-ming,CHEN Xiu-li,LI Yong-mei,ZHANG Yan-ming,LI Ming,CHEN Xiao-han.Application of Suppression Subtractive Hybridization in Shrimp Gene Cloning[J].Progress In Veterinary Medicine,2008,29(4):76-80. |
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| Authors: | ZHAO Yong-zhen JIANG Wei-ming CHEN Xiu-li LI Yong-mei ZHANG Yan-ming LI Ming CHEN Xiao-han |
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| Abstract: | Suppression subtractive hybridization(SSH) technology is an experimental method that sieves and separates unknown differentially expressed genes.It possess some merits of speediness,brief,convenience,sensitivity,speciality,effectiveness and less quantity of the original sample,and so on.SSH was used to construct subtractive cDNA libraries by suppression subtractive hybridization,which was employed to identify differentially expressed genes and their expression profiles in shrimp tissue.Research on differentially expressed immune-relevant genes,virus-resistant genes and other genes,in order to build good base through further studying antiviral mechanism in shrimp,offers an effective channel for antiviral breeding through antiviral genes,and offers a prospect for raising economic effectiveness. |
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| Keywords: | suppression subtractive hybridization shrimp differential expressed gene cDNA libraries |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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