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| 猪流感病毒H1N1分离株HA基因的克隆与序列分析 | |
| 引用本文： | 范锋,倪谷音,毛爱民,姚静,陆春兵,褚小冬,顾伟军.猪流感病毒H1N1分离株HA基因的克隆与序列分析[J].动物医学进展,2008,29(8). |
| 作者姓名： | 范锋 倪谷音 毛爱民 姚静 陆春兵 褚小冬 顾伟军 |
| 作者单位： | 1. 江苏省无锡市动物疫病预防控制中心,江苏无锡,214026 2. 江苏省启东市畜牧兽医站,江苏启东,226000 3. 江阴市畜牧兽医站,江苏江阴,214000 4. 无锡市动物卫生监督所,江苏无锡,214026 5. 无锡市北塘区黄巷兽医站,江苏无锡,214000 |
| 摘 要： | 无锡某猪场发生不同程度的猪呼吸系统疾病.对发病猪采集鼻拭子,经双抗处理后,接种鸡胚,收集24 h后死亡的鸡胚尿囊液.对一株具有血凝性的病毒分离株进行RT-PCR鉴别,结果为H1亚型流感病毒.利用RT-PCR扩增尿囊液中的流感病毒HA基因.经pGEM-T easy载体克隆、序列测定和进化树分析,结果表明,在该猪群中分离获得的该株SIV为古典型H1N1. |
| 关 键 词： | 猪流感病毒 分离 |
Cloning and Sequence Analysis of HA Gene of Swine influenza virus H1N1 |
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| FAN Feng,NI Gu-yin,MAO Ai-min,YAO Jing,LU Chun-bing,CHU Xiao-dong,GU Wei-jun.Cloning and Sequence Analysis of HA Gene of Swine influenza virus H1N1[J].Progress In Veterinary Medicine,2008,29(8). | |
| Authors: | FAN Feng NI Gu-yin MAO Ai-min YAO Jing LU Chun-bing CHU Xiao-dong GU Wei-jun |
| Abstract: | An isolate of influenza virus was isolated from swine which had respiratory disease in a farm in Wuxi city.Subtype was identified from H3 by RT-PCR.The result showed that the isolate was H1N1 subtype.HA gene was then amplified from the niaonangye using the specific primers.The PCR product was subcloned into pGEM-T easy vector by identification with enzymatic restriction.As a result of the sequencing analysis of HA gene,the isolate was characterized as H1N1 serotype by analysis of the phylogenetic tree. |
| Keywords: | H1N1 |
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