| 橙皮苷对3种霉菌毒素处理的HepG2细胞增殖和迁移的影响 |
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| 引用本文： | 晁雨竹,薛皓月,邱思奇,李秋明,沈红.橙皮苷对3种霉菌毒素处理的HepG2细胞增殖和迁移的影响[J].动物医学进展,2020(3):77-81. |
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| 作者姓名： | 晁雨竹 薛皓月 邱思奇 李秋明 沈红 |
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| 作者单位： | 北京农学院动物科学技术学院/动物类国家级实验教学示范中心;北京农学院兽医学（中医药）北京市重点实验室 |
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| 基金项目： | 人才培养质量建设-高水平人才交叉培养-实培计划(市级PXM2018-014207-000022);2019年北京农学院本科生教育管理质量提升项目;动物源食品安全教学团队(BUA2017JG068)。 |
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| 摘 要： | 探讨橙皮苷对3种霉菌毒素处理的HepG2细胞增殖和迁移作用的影响。采用MTT、Griess、细胞划痕愈合试验分别检测细胞活力、细胞分泌NO、细胞迁移率。结果3种毒素对HepG2细胞增殖的影响随毒素浓度的增加呈现先促进后抑制的作用,低浓度DON、OTA(0.04μmol/L和0.08μmol/L),ZEN(<2μmol/L)显著提高HepG2细胞活力及促进增殖,高浓度霉菌毒素明显降低HepG2细胞活力及抑制细胞增殖。与霉菌毒素组比较,橙皮苷组HepG2细胞活力降低及增殖减弱,表示橙皮苷能缓解霉菌毒素对细胞的毒性。与空白对照组比较,霉菌毒素组HepG2分泌NO量显著降低,不同浓度及不同霉菌毒素对NO分泌没有明显影响;与霉菌毒素组比较,橙皮苷组不同浓度DON处理的细胞分泌NO差异不显著。低浓度DON毒素(0.08μmol/L^2μmol/L)处理HepG2细胞24、36、48 h后,DON毒素能极显著促进HepG2细胞迁移,随着霉菌毒素对细胞处理时间延长细胞迁移快,而高浓度DON毒素(10μmol/L、50μmol/L)对促细胞迁移影响显著低于低浓度DON。结果低浓度DON霉菌毒素促进HepG2细胞增殖及迁移,表明DON毒素对HepG2具有潜在的致癌性,橙皮苷能抑制HepG2细胞增殖及缓解霉菌毒素毒性的作用。
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| 关 键 词： | 橙皮苷 霉菌毒素 HEPG2细胞 增殖 迁移 |
Effects of Hesperidin on Proliferation and Migration of HepG2 Cells Treated with Mycotoxin |
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| CHAO Yu-zhu,XUE Hao-yue,QIU Si-qi,LI Qiu-ming,SHEN Hong.Effects of Hesperidin on Proliferation and Migration of HepG2 Cells Treated with Mycotoxin[J].Progress In Veterinary Medicine,2020(3):77-81. |
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| Authors: | CHAO Yu-zhu XUE Hao-yue QIU Si-qi LI Qiu-ming SHEN Hong |
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| Institution: | (Animal Science and Technology College,Beijing University of Agriculture/National Demonstration Center for Experimental animal Education(Beijing University of Agriculture),Beijing 102206,China;Beijing Key Laboratory of Traditional Chinese Veterinary Medicine,Beijing University of Agriculture,Beijing 102206,China) |
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| Abstract: | The experiment was to investigate the effect of hesperidin on the proliferation and migration of HepG2 cells treated with mycotoxin DON.Griess and scratch healing test were used to detect cell viability,NO secretion and cell migration ability.The effects of the three toxins on the proliferation of HepG2 cells were first promoted and then inhibited with the increasing of toxin concentration.Low concentrations of DON,OTA(0.04,0.08μmol/L)and ZEN(<2μmol/L)significantly increased cell viability and proliferation of HepG2 cells,while high concentration of mycotoxin significantly reduced cell viability and inhibited the proliferation of HepG2 cells.Compared with mycotoxin group,HepG2 cells in the hesperidin group showed decreased cell vitality and proliferation,indicating that hesperidin could alleviate the toxicity of mycotoxin to HepG2 cells.Compared with mycotoxin group,there was no significant difference in the secretion of NO from HepG2 cells treated with different concentrations of DON in hesperidin group.After 24,36 and 48 hr treatment of HepG2 cells with low concentration of DON toxin(0.08μmol/L-2μmol/L),DON toxin could significantly promote the migration of HepG2 cells.As the treatment time of mycotoxin on HepG2 cells is prolonged,the migration of HepG2 cells was rapid,while the effect of promoting the migration of HepG2 cells treated with high concentration of DON toxin(10 and 50μmol/L)was significantly lower than that of low concentration DON.The results showed that low concentration of DON mycotoxin promoted the proliferation and migration of HepG2 cells,which indicated that DON toxin could have potential carcinogenicity to HepG2 cells,and hesperidin could inhibit the proliferation of HepG2 cells and alleviate the toxic effects of mycotoxin. |
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| Keywords: | hesperidin mycotoxin HepG2 cell proliferation migration |
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