| 饲粮补充精氨酸对低出生重哺乳仔猪肝脏氧化还原状态和线粒体功能的影响 |
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| 引用本文： | 张浩,陈代文,余冰,何军,虞洁,毛湘冰,罗玉衡,黄志清,罗钧秋,郑萍.饲粮补充精氨酸对低出生重哺乳仔猪肝脏氧化还原状态和线粒体功能的影响[J].动物营养学报,2020(2):654-663. |
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| 作者姓名： | 张浩 陈代文 余冰 何军 虞洁 毛湘冰 罗玉衡 黄志清 罗钧秋 郑萍 |
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| 作者单位： | 四川农业大学动物营养研究所动物抗病营养教育部重点实验室 |
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| 基金项目： | 国家自然科学基金青年项目（31501963) |
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| 摘 要： | 本试验通过研究精氨酸(Arg)对低出生重(LBW)哺乳仔猪机体氧化还原平衡状态和线粒体功能的影响,探讨Arg改善LBW哺乳仔猪生长发育的可能机制。试验选取体况接近、产期一致和胎次相近的初产母猪所产仔猪,4日龄时,选取20头LBW体重(1.16±0.08)kg]和10头正常出生重(NBW)体重(2.07±0.10)kg]仔猪,按体重相近、公母比例一致的原则分为NBW组(饲喂基础饲粮)、LBW组(饲喂基础饲粮)和LBW+Arg组(基础饲粮补充1%Arg)3个组,每组10个重复,每个重复1头猪,人工乳饲养21 d。在第22天,屠宰并收集所有试验猪的血清与肝脏样品,检测生长性能、氧化还原状态与线粒体功能指标。结果表明:1)与NBW仔猪相比,LBW仔猪末重、平均日增重(ADG)和平均日干物质摄入量(ADMI)显著降低(P<0.05),肝脏过氧化氢酶(CAT)活性和ATP含量显著下降(P<0.05),肝脏环氧化酶(COX)ⅠmRNA表达量显著下调(P<0.05),肝脏视神经萎缩症蛋白1(OPA1)mRNA表达量有下调的趋势(P=0.089);2)饲粮补充Arg显著提高LBW仔猪末重、ADG和ADMI(P<0.05),显著提高血清CAT活性和肝脏CAT、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)活性(P<0.05),显著提高肝脏ATP含量(P<0.05),显著上调肝脏GPx1和线粒体融合蛋白1(Mfn1)mRNA表达量(P<0.05),并且有上调肝脏CAT(P=0.056)、COXⅣ(P=0.063)和OPA1(P=0.087)mRNA表达量的趋势。以上研究表明,LBW仔猪肝脏抗氧化能力下降,线粒体功能受阻,生长发育受到抑制;而饲粮补充1%Arg显著提高LBW仔猪肝脏抗氧化能力,改善线粒体功能,提高LBW仔猪生长性能。
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| 关 键 词： | 低出生重 哺乳仔猪 精氨酸 氧化还原状态 线粒体功能 |
Effects of Dietary L-Arginine on Liver Redox Status and Mitochondrial Function of Suckling Piglets with Low Birth Weight |
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| ZHANG Hao,CHEN Daiwen,YU Bing,HE Jun,YU Jie,MAO Xiangbing,LUO Yuheng,HUANG Zhiqing,LUO Junqiu,ZHENG Ping.Effects of Dietary L-Arginine on Liver Redox Status and Mitochondrial Function of Suckling Piglets with Low Birth Weight[J].Acta Zoonutrimenta Sinica,2020(2):654-663. |
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| Authors: | ZHANG Hao CHEN Daiwen YU Bing HE Jun YU Jie MAO Xiangbing LUO Yuheng HUANG Zhiqing LUO Junqiu ZHENG Ping |
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| Institution: | (Key Laboratory for Animal Disease?Resistance Nutrition of Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Ya’an 625014,China) |
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| Abstract: | This study investigated the effects of L?arginine(Arg)on liver redox balance status and mitochon?drial function of suckling piglets with low birth weight(LBW)aiming to explore the possible mechanism of Arg,which improved the growth and development of LBW suckling piglets.Piglets from primary sows with similar body condition,delivery period and parity were selected,and at 4 days of age,ten normal birth weightNBW,body weight of(2.07±0.10)kg]piglets and twenty LBWbody weight of(1.16±0.08)kg]pig?lets were selected and divided into 3 groups with 10 replicates per group and 1 pig per replicate according to the similar body weight and consistent proportion of males and females.NBW piglets were fed a basal diet(a kind of artificial milk,NBW group),and LBW piglets were fed the basal diet(LBW group)or the basal diet sup?plemented with 1%Arg(LBW+Arg group).The experiment lasted for 21 days.At day 22,all piglets were slaughtered,then serum and liver samples were collected and growth performance,redox status,and mitochon?drial function were determined.The results showed as follows:1)compared with NBW piglets,the final weight,average daily gain(ADG)and average daily dry matter intake(ADMI)of LBW piglets were signifi?cantly decreased(P<0.05),the liver catalase(CAT)activity and ATP content were also significantly reduced(P<0.05),the liver cyclooxygenase(COX)ⅠmRNA expression level was significantly down?regulated(P<0.05),and the liver optic atrophy protein 1(OPA1)mRNA expression level was tended to be down?regulated(P=0.089).2)Dietary Arg significantly improved the final weight,ADG and ADMI(P<0.05),significant?ly enhanced serum CAT activity and liver activity of CAT,superoxide dismutase(SOD)and glutathion perox?idase(GPx)(P<0.05),significantly increased liver ATP content(P<0.05),significantly up?regulated the mRNA expression levels of liver GPx1 and mitofusin 1(Mfn1)(P<0.05),and tended to up?regulate the mR?NA expression levels of liver CAT(P=0.056),COXⅣ(P=0.063)and OPA1(P=0.087).In summary,LBW piglets exhibit lower liver antioxidant capacity and blocked mitochondrial function,which leads to im?paired growth and development of piglets;while 1%Arg supplementation promotes growth performance through enhancing antioxidant capacity and improving mitochondrial function in liver of LBW piglets. |
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| Keywords: | low birth weight suckling piglets arginine redox status mitochondrial function |
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