| 3株临床分离的猪流行性腹泻病毒S基因克隆及序列分析 |
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| 引用本文： | 张辛铭,杨顺利,马雪婷,曹钰莹,何雪阳,蔡建平,尹双辉.3株临床分离的猪流行性腹泻病毒S基因克隆及序列分析[J].动物医学进展,2020(7):16-20. |
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| 作者姓名： | 张辛铭 杨顺利 马雪婷 曹钰莹 何雪阳 蔡建平 尹双辉 |
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| 作者单位： | 中国农业科学院兰州兽医研究所;江苏省动物重要疫病与人兽共患病防控协同创新中心 |
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| 基金项目： | 中国农业科学院创新工程支持项目(CAAS-ASTIP-2014-LVRI-09)。 |
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| 摘 要： | 对临床分离鉴定的3株猪流行性腹泻病毒(PEDV)的S基因进行克隆及序列分析,对丰富我国PEDV的遗传衍化特征具有重要意义。S基因序列分析发现,JX18毒株与CV777亲缘关系较近,核苷酸同源性为99.71%,氨基酸同源性95.7%,属于G1-b进化分支。JX18毒株与CV777经典毒株S蛋白氨基酸序列相比较,不存在氨基酸的插入或缺失,在中和表位区及已鉴定抗原表位区仅有一处氨基酸突变。SD18、JingTai18与PEDV CV777亲缘关系较远,核苷酸同源性分别为93.64%和92.64%,S蛋白氨基酸序列同源性分别为93%和93.5%。SD18和JingTai18位于G2-b进化分支。SD18与JingTai18株与CV777经典毒株相比,S蛋白氨基酸序列中存在插入缺失及多位点的氨基酸突变。研究结果为进一步了解我国PEDV流行及变异情况提供了数据支持。
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| 关 键 词： | 猪流行性腹泻病毒 S基因 生物信息学分析 遗传变异 |
Cloning and Sequence Analysis of S Gene of Three Porcine Epidemic Diarrhea Viruses Isolated from Clinical Samples |
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| ZHANG Xin-ming,YANG Shun-li,MA Xue-ting,CAO Yu-ying,HE Xue-yang,CAI Jian-ping,YIN Shuang-hui.Cloning and Sequence Analysis of S Gene of Three Porcine Epidemic Diarrhea Viruses Isolated from Clinical Samples[J].Progress In Veterinary Medicine,2020(7):16-20. |
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| Authors: | ZHANG Xin-ming YANG Shun-li MA Xue-ting CAO Yu-ying HE Xue-yang CAI Jian-ping YIN Shuang-hui |
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| Institution: | (National Key Laboratory of Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu,730046,China;Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou,Jiangsu,225009,China) |
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| Abstract: | In this study,the cloning and sequence analysis of the S gene of three porcine epidemic diarrhea viruses(PEDV)identified in the clinical samples were of great significance for enriching the genetic evolution characteristics of PEDV in China.Sequence analysis of S gene revealed that the JX18 strain is closely related to CV777,with nucleotide homology of 99.71%and amino acid homology of 95.7%,belonging to the G1-b evolutionary branch.Compared with the protein sequence of the CV777 classic strain,the JX18 strain does not have an amino acid insertion or deletion,and there is only one amino acid mutation at the neutralizing antigentic epitope and the epitope.SD18,JingTai18 are far away from PEDV CV777,nucleotide homologies are 93.64%and 92.64%,respectively,and protein sequence homologies are 93%and 93.5%,respectively.SD18 and JingTai18 are located in the G2-b evolutionary branch.Compared with the CV777 classic strain,SD18 and JingTai18 strains have insertions deletions and multiple amino acid mutations in the protein sequences.The research results provided data support for further understanding the prevalence and variation of PEDV in China. |
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| Keywords: | Porcine epidemic diarrhea virus S gene bioinformatics analysis genetic variation |
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