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不同番茄品种对5种植物双生病毒的抗病性鉴定
引用本文:矫晓阳,周雪平,杨悦俭,谢艳.不同番茄品种对5种植物双生病毒的抗病性鉴定[J].植物病理学报,2013,43(6):655-658.
作者姓名:矫晓阳  周雪平  杨悦俭  谢艳
作者单位:1. 浙江大学生物技术研究所,杭州 310058;
2.浙江省农业科学院蔬菜研究所,杭州 310021
基金项目:公益性(农业)行业科研专项(201003065);国家自然科学基金资助项目(31171814)
摘    要: 由粉虱传双生病毒引起的番茄曲叶病[1]在我国最初仅分布在海南、云南、广东和广西,自2006年上海市和浙江省先后在番茄上发现番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)以来,该病害蔓延迅速,在多个省份的番茄上暴发成灾[2]。引起番茄曲叶病害的病原较复杂,在我国其主要病原为TYLCV、中国番木瓜曲叶病毒(Papaya leaf curl China virus, PaLCuCNV)、中国番茄黄化曲叶病毒(Tomato yellow leaf curl China virus, TYLCCNV)、泰国番茄黄化曲叶病毒(Tomato yellow leaf curl Thailand virus, TYLCTHV)和台湾番茄曲叶病毒(Tomato leaf curl Taiwan virus, ToLCTWV)[2~5],而浙江省的主要病原为TYLCV和ToLCTWV。选育抗病品种是防治番茄黄化曲叶病最有效的手段。了解番茄品种对不同双生病毒的抗性,对因地制宜布局抗病品种具有重要意义。浙杂502、浙粉701、浙粉702是浙江省大规模种植的番茄品种,为了解这些品种对上述5种病毒的抗性,本研究利用5种病毒的侵染性克隆,在人工接种条件下,综合评定分析这3个番茄品种的抗病指标。


Identification for resistance of tomato varieties against geminiviruses
JIAO Xiao-yang,ZHOU Xue-ping,YANG Yue-jian,XIE Yan.Identification for resistance of tomato varieties against geminiviruses[J].Acta Phytopathologica Sinica,2013,43(6):655-658.
Authors:JIAO Xiao-yang  ZHOU Xue-ping  YANG Yue-jian  XIE Yan
Institution:1.Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China;
2.Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Abstract:Based on the agro-inoculation method, the resistant levels of 3 tomato varieties, Zheza 502, Zhefen 701 and Zhefen 702, against Papaya leaf curl China virus (PaLCuCNV), Tomato yellow leaf curl virus (TYLCV), Tomato yellow leaf curl China virus (TYLCCNV), Tomato yellow leaf curl Thailand virus (TYLCTHV) and Tomato leaf curl Taiwan virus (ToLCTWV) were tested in an insect-free greenhouse. The variety Hongbaoshi was set as the susceptible control, and healthy tomatoes were set as negative controls. After inoculation, symptom development was investigated including the initial time of symptom, symptoms, disease incidence and disease index, and PCR was performed to test the rate of infection. Zheza 502 possessed of the best resistance against 5 begomoviruses and followed by Zhefen 701 and Zhefen 702 with a certain resistance. Each tomato variety had different resistant levels against 5 begomoviruses.
Keywords:tomato varieties  begomoviruses  infectious clone  resistance identification  
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