| katExoo基因缺失突变对水稻白叶枯病菌H2O2抗性和致病性的影响 |
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| 引用本文： | 王努,吴茂森,田芳,陈华民,何晨阳.katExoo基因缺失突变对水稻白叶枯病菌H2O2抗性和致病性的影响[J].植物病理学报,2013,43(6):615-621. |
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| 作者姓名： | 王努 吴茂森 田芳 陈华民 何晨阳 |
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| 作者单位： | 中国农业科学院植物保护研究所，植物病虫害生物学国家重点实验室，北京100193 |
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| 基金项目： | 国家“973”计划项目课题(2011CB100701)资助 |
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| 摘 要： | 为了揭示过氧化氢酶基因katExoo在水稻白叶枯病菌(Xanthomonasoryzaepv.oryzae，Xoo)过氧化氢(H2O2)抗性和致病性中的功能，本研究构建了基因缺失突变体ΔkatExoo，测定了突变体的H2O2抗性、过氧化氢酶(CAT)活性、在离体培养条件下的生长速率以及对水稻的致病性。用标记基因置换法获得了ΔkatExoo突变体，其保守的CAT结构域(GATase1_catalase和catalase_clade_2)被GmR片段所替换。katExoo基因缺失突变并不导致病菌的H2O2抗性和CAT活性降低或丧失，反而在一定程度上使之增强和升高。ΔkatExoo离体生长量显著降低，水稻接种叶片病斑明显缩短、在叶组织内的种群量下降。表明基因缺失突变显著地影响了病菌的生长、定殖和致病性。本研究结果为“KatExoo可能是Xoo的一个毒性因子”的假设提供了遗传学证据。
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| 关 键 词： | 水稻白叶枯病菌 katExoo H2O2抗性 致病性 |
| 收稿时间： | 2012-07-15 |
Effects of gene deletion of katExoo encoding putative catalase on resistance to hydrogen peroxide and pathogenicity of Xanthomonas oryzae pv. oryzae |
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| WANG Nu,WU Mao sen,TIAN Fang,CHEN Hua min,HE Chen yang.Effects of gene deletion of katExoo encoding putative catalase on resistance to hydrogen peroxide and pathogenicity of Xanthomonas oryzae pv. oryzae[J].Acta Phytopathologica Sinica,2013,43(6):615-621. |
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| Authors: | WANG Nu WU Mao sen TIAN Fang CHEN Hua min HE Chen yang |
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| Institution: | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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| Abstract: | To reveal the role of katExoo encoding putative catalase (CAT) in H2O2 resistance and pathogenicity of Xanthomonas oryzae pv. oryzae, the gene deletion and assays for H2O2 resistance, CAT activity, in vitro growth, and virulence on rice of the ΔkatExoo mutant were performed in this study. The mutant was generated from Xoo wildtype strain PXO99A through marker exchange, in which the conserved domain of CAT (GATase1_catalase and catalase_clade_2) was replaced by GmR. Significant changes in increased H2O2 resistance and higher CAT activity were found in the mutant compared to PXO99A. Moreover, remarkable decreases in bacterial growth rate in vitro and in planta of ΔkatExoo and diseased leaf lesion of rice were observed. Therefore, katExoo has been shown to play a critical role in bacterial growth, colonization and pathogenicity on rice. This work partially provides first genetic evidence to support the hypothesis that KatExoo might function as a virulence factor in Xoo. |
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| Keywords: | Xanthomonas oryzae pv oryzae katExoo H2O2 resistance pathogenicity |
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