| 玉米穗粒腐病接种技术及品种抗病性鉴定 |
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| 引用本文： | 马秉元,李亚玲,龙书生,李多川.玉米穗粒腐病接种技术及品种抗病性鉴定[J].植物保护学报,1999,26(2):121-124. |
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| 作者姓名： | 马秉元 李亚玲 龙书生 李多川 |
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| 作者单位： | 陕西省农业科学院植物保护研究所，陕西省农业科学院植物保护研究所，陕西省农业科学院植物保护研究所，陕西省农业科学院植物保护研究所 杨凌 712100，杨凌 712100，杨凌 712100，杨凌 712100 |
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| 基金项目： | 陕西省“八五”攻关课题，陕西省科委立项资助 |
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| 摘 要： | 以玉米穗粒腐病的优势病原菌串珠镰刀菌Fusarium moniliforme为接种菌源,采用4种接种方法进行比较,以针刺果穗较深处(籽粒与穗轴间)发病最重,花丝喷雾法发病最轻;接种时期以玉米乳熟期接种为好;接种浓度10×40倍下每视野60个分生孢子;接种量以每果穗接种2.5ml孢子悬浮液发病最重。同时规范了病情分级标准及品种抗、感程度划分标准。采用规范程序,对63份玉米自交系及杂交种进行了抗病性鉴定。
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| 关 键 词： | 玉米 穗粒腐病 接种 品种 抗病性 病害 |
INOCULATION TECHNIQUE OF CORN KERNEL OR EAR ROT AND IDENTIFICATION OF VARIETY RESISTANCE |
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| Ma Bingyuan,Li Yaling,Long Shusheng and Li Duochuan.INOCULATION TECHNIQUE OF CORN KERNEL OR EAR ROT AND IDENTIFICATION OF VARIETY RESISTANCE[J].Acta Phytophylacica Sinica,1999,26(2):121-124. |
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| Authors: | Ma Bingyuan Li Yaling Long Shusheng and Li Duochuan |
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| Institution: | Institute of Plant Protection, Shaanxi Academy of Agricultural Sciences, Yangiing 712100,Institute of Plant Protection, Shaanxi Academy of Agricultural Sciences, Yangiing 712100,Institute of Plant Protection, Shaanxi Academy of Agricultural Sciences, Yangiing 712100 and Institute of Plant Protection, Shaanxi Academy of Agricultural Sciences, Yangiing 712100 |
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| Abstract: | Four inoculating methods with Fusarium moniliforme Sheld as a preponderant pathogen of corn kernel or ear rot were compared. The results indicated that injecting conidia suspension at the deeper part between kernel tips and cob caused the highest incidence while spraying the suspension onto filaments caused the lightest incidence. The optimal time of inoculation was at milk stage. 2. 5ml conidia suspension with 60 conidia in one field under microscope at 10x40 times amplification showed the most effective result. Meanwhile, disease degrees as well as evaluating standards of resistance or susceptibility of corn varieties were established in this study. The disease resistance of 63 corn self-lines and hybrids were identified with a standard procedure. |
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| Keywords: | corn kernel or ear rot technique of inoculation disease resistance of varieties |
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