| 植物乳杆菌IMAUl0116产生抗娄地青霉活性物质培养基的优化 |
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| 引用本文： | 戚薇,石景,尹娅凤,王海宽.植物乳杆菌IMAUl0116产生抗娄地青霉活性物质培养基的优化[J].安徽农业科学,2011(19):11937-11939. |
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| 作者姓名： | 戚薇 石景 尹娅凤 王海宽 |
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| 作者单位： | 工业发酵微生物教育部重点实验室,天津科技大学生物工程学院,天津300457 |
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| 基金项目： | 国家自然科学基金项目（20876116、30900961）;乳品生物技术与工程教育部重点实验室开放课题基金项目. |
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| 摘 要： | 目的]筛选对娄地青霉有较强抑菌活性的植物乳杆菌并提高其抗真菌活性。方法]将活化后的植物乳杆菌IMAUl0116接种到MRs液体培养基中培养，采用双层平板点接法评价其抗娄地青霉菌的活性。结果]单因素试验表明，以葡萄糖为碳源时，植物乳杆菌IMAUl0116对娄地青霉的抑制效果最好；植物乳杆菌以麦芽浸粉为氮源时，抑茵效果最好；抗真菌乳酸茵产生抗茵物质的最佳生长因子为玉米浆粉。植物乳杆菌IMAUl0116最佳培养基组成为：葡萄糖20/L＋麦芽浸粉20g／L＋玉米浆粉7/L＋适量无机盐。用优化培养基培养的植物乳杆菌IMAUl0116抑制娄地青霉菌产生的抑菌圈为18．6mm，用MRs培养基所产生的抑菌圈为15．0mm。结论]植物乳杆菌IMAUl0116对娄地青霉有很好的抑制作用，有望在食品及饲料添加剂领域获得较好的应用。
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| 关 键 词： | 植物乳杆菌 培养基优化 抗真菌 娄地青霉 |
Optimization of Medium for LactobaciUus plantarum IMAU10116 Producing Active Compound against PeniciUium roqueforti |
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| Institution: | Qi Wei et al ( Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457 ) |
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| Abstract: | Objective ] The study aimed to screen out Lactobacillus plantarum possessing higher inhibitory activity against Penicillium roque- forti and enhance its antifungal activity. Method] The activated L. plantarum IMAU10116 was innoculated MRS liquid medium for culturing and double-layer plate point inoculation was used to evaluate its activity against P. roqueforti. Result] The single-factor experiments showed＂ that when glucose was used as carbon source, the inhibitory effect of L. plantarum IMAU101 t6 against P. roqueforti was best; when malt ex- tract powder was used as nitrogen source, its inhibitory effect was best ; the optimum growth factor for antifungal lactohacilus producing anti- fungal substance was powder of corn steep liquor. The optimum medium composition of L. plantarum IMAU10116 was 20 g/L glucose ＋ 20 g/L malt extract powder ＋ 7 g/L powder of corn steep liquor ＋ quantum sufficit mineral salt. The diameter of inhibition zone of L. plantarum IMAU10116 cultured with optimized medium against P. roqueforti was 18.6 mm and that of L. plantarum IMAU10116 cultured with MRS me- dium was 15.0 ram. Conclusion] L. plantarum IMAUI0116 had very good inhibition activity against P. roqueforti and it was hopeful to get better application in food and forage additive domain. |
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| Keywords: | Lactobacillus plantarum Medium optimization Antifungal Penicillium roqueforti |
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