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香蕉枯萎病拮抗菌的筛选及抑菌作用研究
引用本文:王芳,李静,陈云.香蕉枯萎病拮抗菌的筛选及抑菌作用研究[J].河南农业科学,2009(8).
作者姓名:王芳  李静  陈云
作者单位:佛山科学技术学院,广东,佛山,528231
基金项目:佛山市科技发展专项基金 
摘    要:从香蕉园土壤中分离和筛选得到33株对香蕉枯萎病菌(Fusari um ox ysporumf.sp.cubense)有抑制作用的拮抗菌,对其进行了抑制病原菌菌丝生长和孢子萌发的试验,结果表明:拮抗菌株G1、G3、F1、F3I、1发酵液对香蕉枯萎病菌的生长有显著的抑制作用,在平板上产生的抑菌圈直径为10.33~48.33mm,抑菌效果持续稳定;对孢子的萌发抑制率为92.50%~98.22%,拮抗菌的抑制作用表现为抑制病菌分生孢子的萌发,致使孢子芽管扭曲。

关 键 词:香蕉枯萎病  拮抗菌  作用机制

Screening of Antagonistic Bacteria against Fusarium oxysporumf.sp.cubense in Banana
WANG Fang,LI Jing,CHEN Yun.Screening of Antagonistic Bacteria against Fusarium oxysporumf.sp.cubense in Banana[J].Journal of Henan Agricultural Sciences,2009(8).
Authors:WANG Fang  LI Jing  CHEN Yun
Abstract:Thirty-three antagonistic bacterium stains against Fusarium oxysporum f.sp.cubense were isolated from the soils of banana,and tested about their inhibitive efficiency to the hyphal growth and conidial germination.The result showed that the antagonistic bacterium strains G1,G3,F1,F3,and I1 had significant inhibition to Fusarium oxysporum f.sp.cubense,the induced zone of inhibition ranged from 10.33-48.33mm in diameter on PDA plates,and the inhibition rates to conidial germination ranged from 92.50% to 98.22%.The studies on the action mechanisms of the antagonistic bacteria demonstrated that they could inhibit conidial germination and distort the germ tubes of the conidia of the pathogens.
Keywords:Fusarium oxysporum f  sp  cubense  Antagonistic bacterium  Action mechanism
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