| 超声波法提取猴头下脚料多糖工艺研究 |
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| 引用本文： | 柴军红,龚振杰,杨春文,吕娜,柴小军.超声波法提取猴头下脚料多糖工艺研究[J].安徽农业科学,2010,38(11):5874-5876. |
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| 作者姓名： | 柴军红 龚振杰 杨春文 吕娜 柴小军 |
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| 作者单位： | |
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| 基金项目： | 科技部科技人员服务企业项目，牡丹江市科技局攻关项目，黑龙江省新世纪教学改革工程项目 |
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| 摘 要： | 目的]综合利用猴头下脚料和残次菇,筛选出超声波法提取多糖的最佳工艺。方法]通过粉碎、超声提取、浓缩、Sevag法除蛋白、醇沉纯化等得到多糖,采用硫酸-蒽酮法测定多糖含量。在设计单因素试验优化多糖提取工艺的基础上,采用正交试验确定超声波法提取猴头下脚料多糖的最佳工艺。结果]pH值在5.0～6.5范围内,经正交试验所得超声波法提取猴头下脚料中多糖的最佳工艺为：料液比（体积比）为1∶70,超声功率为最大功率的80%,提取时间是45min,提取温度为60℃,此时多糖提取率可达9.56%。结论]该工艺条件综合利用下脚料和残次菇,多糖得率较高,有一定的开发利用价值。
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| 关 键 词： | 猴头下脚料 超声波 提取 多糖 |
Study on Extraction Technology of Intracellular Polysaccharide from Byproduct of Hericium erinaceus by Ultrasonic Wave |
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| Institution: | CHAI Jun-hong et al (Department of Biology,MuDanJiang Teachers College,Mudanjiang,Heinongjiang 157012) |
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| Abstract: | Objective] To determine the optimum ultrasonic wave extraction technology of polysaccharide from byproduct of Hericium erinaceus. Method] Polysaccharide was obtained by crushing,ultrasonic extraction,concentration,sevag method,and alcohol extraction,and polysaccharide contents were determined by the anthrone-sulfuric method. Based on the polysaccharide extraction process of optimization of single-factor test,using orthogonal test to determine the optimum extraction technology of intracellular polysaccharide from by product of Hericium erinaceus by ultrasonic wave.Result] While pH value was from 5 to 6.5,the optimum technology of extracting polysaccharide from byproduct of Hericium erinaceus by ultrasonic method: solid-liquid ratio (volume ratio) was 1∶70; ultrasonic power was 80% of the maximum power;extraction time was 45 min,extraction temperature was 60 ℃,and the extraction rate of polysaccharide from byproduct of Hericium erinaceus was up to 9.56%. Conclusion] Extraction technology of polysaccharide from byproduct of Hericium erinaceus had a certain degree of development and utilization value,it provided the theoretic basis for the comprehensive utilization of the wastes and byproduct of Hericium erinaceus. |
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| Keywords: | Byproduct of Hericium erinaceus Ultrasonic wave Extraction Polysaccharide |
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