| AA肉种鸡胚胎性疾病的病原学研究及主要病原的PCR 检测 |
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| 引用本文： | 刘红芹,朱瑞良,孙秋艳,胡晓娜,毕建敏,刘红珍,李新苍,王伟.AA肉种鸡胚胎性疾病的病原学研究及主要病原的PCR 检测[J].农业生物技术学报,2006,14(5):646-651. |
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| 作者姓名： | 刘红芹 朱瑞良 孙秋艳 胡晓娜 毕建敏 刘红珍 李新苍 王伟 |
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| 作者单位： | 山东农业大学动物科技学院,泰安,271018 |
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| 基金项目： | 中国博士后科学基金;山东省优秀中青年科学家科研奖励基金;山东农业大学校科研和教改项目;国家自然科学基金 |
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| 摘 要： | 2004年6月份以来，山东某一大型AA父母代肉种鸡场孵化场出现胚胎死亡、弱雏增多、孵出的雏鸡30日龄左右免疫应答不良、生长缓慢等症状。为了探明其病因，作者通过流行病学调查和病原学检查，结果表明造成该病的主要原因是由禽波氏杆菌(Bordetella avium)、沙门氏菌(Salmonella)、大肠杆菌(Escherichia coli)、支原体(滑液囊和鸡败血支原体)及鸡传染性贫血病病毒（CIAV）和病毒性关节炎病毒（VAV）共感染种鸡后，由种蛋垂直传播到鸡胚、雏鸡造成的。同时对其主要病原禽波氏杆菌进行了16S rRNA基因的扩增，结果扩增出783 bp的目的条带; 通过PCR对CIAV VP3基因及VAV S1基因进行扩增，结果分别扩增出582和532 bp的目的条带，其PCR产物均分别能与相应的探针杂交，呈现阳性反应。
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| 关 键 词： | AA肉种鸡 胚胎性疾病 禽波氏杆菌 16S rRNA 免疫抑制病 核酸探针 |
| 文章编号： | 1006-1304（2006）05-0646-06 |
| 收稿时间： | 2005-12-16 |
| 修稿时间： | 2006-02-27 |
Pathogenic Studies on the Diseases of Avian Embryo in AA Broiler Breeder and the Detection of Its Main Pathogens by PCR |
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| LIU Hong-qin,ZHU Rui-liang,SUN Qiu-yan,HU Xiao-na,BI Jian-min,LIU Hong-zhen,LI Xin-cang,WANG Wei.Pathogenic Studies on the Diseases of Avian Embryo in AA Broiler Breeder and the Detection of Its Main Pathogens by PCR[J].Journal of Agricultural Biotechnology,2006,14(5):646-651. |
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| Authors: | LIU Hong-qin ZHU Rui-liang SUN Qiu-yan HU Xiao-na BI Jian-min LIU Hong-zhen LI Xin-cang WANG Wei |
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| Institution: | College of Animal Science and Technology, Shandong Agricultural University, Taian 2 71018,China |
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| Abstract: | A kind of disease appeared in one large-scale AA broiler breeder farm in Shandong Province with the symptom of embryo death, increase of weak chicken, ineffective immunity, stunted growth of 30 days chicken since June, 2004. Based on the epidemiology investigation and the pathogenic studies, it was showed that the co-infection of Bordetella avium, Salmonella, Escherichia coli, mycoplasma, Chicken infectious anemia virus(CIAV) and Viral arthritis virus(VAV) was the major cause of the disease. These pathogens spread vertically from the eggs to the embryos. The 16S rRNA gene of Bordetella avium, CIAV VP3 gene and VAV S1 gene were amplified by PCR and 783 582 and 532 bp specific bands were acquired respectively. The PCR products of CIAV VP3 gene and VAV S1 gene could hybridize with the corresponding probe and appear positive reaction. |
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| Keywords: | AA broiler breeder disease of embryo Bordetella avium 16S rRNA immunerepress disease nucleic acid probe |
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