| 草莓花药培养植株再生率影响因素分析 |
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| 引用本文： | 顾玉成,向发云,曾祥国,冯小明,吴金平,吴润玲,宋志红.草莓花药培养植株再生率影响因素分析[J].安徽农业科学,2009,37(3):1035-1036. |
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| 作者姓名： | 顾玉成 向发云 曾祥国 冯小明 吴金平 吴润玲 宋志红 |
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| 作者单位： | 湖北省农业科学院经济作物研究所,湖北武汉,430064;湖北省农业科学院经济作物研究所,湖北武汉,430064;湖北省农业科学院经济作物研究所,湖北武汉,430064;湖北省农业科学院经济作物研究所,湖北武汉,430064;湖北省农业科学院经济作物研究所,湖北武汉,430064;湖北省农业科学院经济作物研究所,湖北武汉,430064;湖北省农业科学院经济作物研究所,湖北武汉,430064 |
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| 基金项目： | 湖北省农业科技成果转化项目，湖北省农业科技创新中心资助项目 |
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| 摘 要： | 目的]提高草莓花药培养效率。方法]以草莓品种“丰香”为材料，研究了不同条件对诱导花药愈伤组织和绿苗分化的影响。结果]8℃预处理3d，草莓花药的愈伤组织诱导率可达64.8％，显著高于处理5d（46．5％）和不经低温处理（40．3％）的诱导率。22℃和25℃培养时，愈伤组织诱导率分别为61．30％和68．36％，前者的愈伤组织优于后者。激素组合BA 0．2mg／L＋KT 1．0mg／L＋NAA 2．0mg／L的出愈率达61．57％，且愈伤质量较好。黄绿色颗粒状、绿色致密、淡黄色蓬松等3种愈伤组织分别占11．5％、49．0％、39．5％。愈伤组织在激素组合ZT 2．0mg／L＋BA 0．5mg／L＋NAA 0．2mg／L中的分化率最高，可达11．28％。生根培养后，花培植株生根率达98％。结论]得到了草莓花药培养的适宜条件。
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| 关 键 词： | 草莓 花药培养 植株再生 |
Effects of Several Factors on Regeneration of Fengxiang Strawberry in Anther Culture |
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| Institution: | GU Yu-cheng et al (Economic Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan, Hubei 430064) |
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| Abstract: | Objective] The study was to improve the culture efficiency of strawberry anther.Method] With strawberry variety Fengxiang as the tested material,the effect of different condition on the induction of anther callus and differentiation of green plantlet was investigated.Result] The induction rate of strawberry anther callus in the pretreatment for 3 d at 8 ℃ could reached 64.8%,being obviously higher than that in treatment for 5 d(46.5%) and that in the treatment without low temperature(40.3%).When the cultured was at 22 ℃ and 25 ℃,the induction rate of callus was 61.3% and 68.36% resp.and the callus of the former was better than that of the latter.The callus rate from the hormone combination of BA 0.2 mg/L + KT 1.0 mg/L +NAA 2.0 mg/L was 61.57% with a better quality. 3 kinds of callus such as the yellow-green granular,green and compact,slight yellow fluffy accounted for 11.5%,49.0% and 39.5% resp.The differentiation rate of callus from hormone combination of ZT 2.0 mg/L +BA 0.5 mg/L +NAA 0.2 mg/L was the highest,being 11.28%.After rooting culture,the rooting rate of anther culture plant was 98%.Conclusion] The optimum condition for the strawberry anther culture was obtained. |
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| Keywords: | Strawberry Anther Culture Plant regeneration |
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