| 果寡糖对猪粪便细菌群作用下L-色氨酸代谢的影响 |
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| 引用本文： | 夏枚生,胡彩虹,许梓荣.果寡糖对猪粪便细菌群作用下L-色氨酸代谢的影响[J].中国畜牧杂志,2004,40(1):11-13. |
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| 作者姓名： | 夏枚生 胡彩虹 许梓荣 |
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| 作者单位： | 浙江大学动物分子营养学教育部重点实验室;浙江大学饲料科学研究所,浙江杭州310029 |
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| 摘 要： | 本试验研究了果寡糖对猪粪便细菌群作用下L -色氨酸代谢的影响。将 10 % (W/V)粪水厌氧分装于无菌瓶中 ,分成 4组 :1组 (对照 )添加 2 5 0 μmol/LL -色氨酸 ;2～ 4组 :分别在对照组的基础上添加 0 .5 % ,1.0 %和 1.5 %果寡糖。 38℃、厌氧培养。在培养期的不同时间点 (0 ,2 ,4 ,6 ,8,12 ,18,2 4h)分别从各瓶中取 1mL培养液 ,分析各培养液中吲哚类物质含量。培养 2 4h后 ,分析各培养液中细菌学指标和pH。结果表明 :含L -色氨酸的体外培养液中添加 0 .5 % ,1.0 %和 1.5 %果寡糖 ,培养 2 4h后 ,使粪臭素浓度、吲哚 - 3-乙酸峰值和pH值均显著降低。添加1.0 %和 1.5 %果寡糖显著降低色氨酸降解率和粪臭素相对产率 ,显著提高吲哚相对产率 ,显著降低梭菌和大肠杆菌数 ,显著增加双歧杆菌和总厌氧菌数。
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| 关 键 词： | 畜牧学 色氨酸代谢 果寡糖 粪臭素 吲哚 |
| 文章编号： | 0258-7033(2004)01-0011-03 |
| 修稿时间： | 2002年12月31 |
Effects of Fructooligosaccharide on the Metabolism of L-tryptophan Mixed Population of Pig Fecal Bacteria |
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| XIA Mei-sheng,HU Cai-hong,XU Zi-rong.Effects of Fructooligosaccharide on the Metabolism of L-tryptophan Mixed Population of Pig Fecal Bacteria[J].Chinese Journal of Animal Science,2004,40(1):11-13. |
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| Authors: | XIA Mei-sheng HU Cai-hong XU Zi-rong |
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| Abstract: | An in vitro study was conducted to examine the effects of fructooligosaccharide (FOS) at levels of 0.5%, 1.0% and 1.5% on conversion of L-tryptophan to skatole and indole by a mixed bacterial population from the large intestines of pigs. Microbial suspensions were anaerobically incubated at 38℃ for 24 h. Samples were periodically removed for determination indole compounds. After 24 h incubation, microbial populations and pH in each culture media were analyzed. Addition of 0.5%, 1.0% and 1.5% FOS to the slurries with L-tryptophan significantly decreased the skatole concentration, the peak value of indole-3-acetic acid and the medium pH. The viable counts of Bifidobacterium were significantly higher than those of control. In addition, 1.0% and 1.5% FOS supplemenation significantly decreased the rate of tryptophan degradation and the relative rate of skatole production. The relative rate of indole production was significantly increased. The viable counts of Clostridium and Escherichia coli were significantly reduced. The total viable counts of anaerobes were significantly increased. |
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| Keywords: | Animal science Metabolism of tryptophan Fructooligosaccharide Skatole Indole |
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