| 绿色木霉降解稻草秸杆产葡萄糖工艺优化研究 |
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| 引用本文： | 郑颖洁,余勃,陆豫.绿色木霉降解稻草秸杆产葡萄糖工艺优化研究[J].安徽农业科学,2010,38(13):6903-6905,6928. |
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| 作者姓名： | 郑颖洁 余勃 陆豫 |
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| 作者单位： | 1. 南昌大学环境科学与工程学院,江西南昌,330047;南昌大学中德联合研究院南昌市生物化工重点实验室,江西南昌,330047
2. 南昌大学中德联合研究院南昌市生物化工重点实验室,江西南昌,330047 |
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| 摘 要： | 目的]采用响应曲面法对绿色木霉（Trichodermaviride）降解稻草秸杆产葡萄糖的工艺进行优化研究。方法]首先通过Plackett-Burman试验从7个发酵参数中筛选出影响葡萄糖产量的3个主要因素,即发酵时间、pH值和料水比。然后利用Box-Behnken试验设计对此3个因素的最佳水平和交互作用进行研究,通过对试验结果的响应面分析得出降解秸秆产葡萄糖的最佳发酵条件。结果]绿色木霉发酵降解秸秆产葡萄糖的最佳发酵条件为：发酵时间2d,pH值为4.5,料水比2.5%,在此条件下,葡萄糖产量可达191.70mg/g。与优化前相比,葡萄糖产量提高了137.58%。结论]该研究提高了将稻草纤维素转化成葡萄糖的转化率,降低了生产成本,为后续发酵生产燃料乙醇打下了良好的基础。
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| 关 键 词： | 绿色木霉 稻草秸秆 响应曲面法 葡萄糖 |
Study on Optimization of Conversion of Straw Stalk into Glucose by Trichoderma viride |
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| Institution: | ZHENG Ying-jie et al (Department of Environment Science and Engineering,Nanchang University,Nanchang,Jiangxi 330047) |
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| Abstract: | Objective] The process parameters for glucose production by Trichoderma viride fermentate straw stalk were optimized based on response surface analysis. Method] First,3 important factors affecting glucose production,which identified by screening method of Plackett- Burman were time,pH value and straw stalk concentration from 7 factors. Then Box- Behnken design were adopted to further investigate optimization level and mutual interaction between variables of 3 factors. The optimal conditions of glucose production by Trichoderma viride were obtained by response surface methodology. Result] The experimental results showed that pH velaue and straw stalk consentration had an individual significant influence on glucose production,and the glucose production up to 191.7 mg/g at the optimal conditions that fermentatio time was 2 days,pH value was 4.5 and straw stalk concentration was 2.5%. Comparing with glucose production before optimization,the production yield improved 137.58%. Conclusion] The study can improve the conversion rate of glucose production by rice straw cellulose,reduce the production cost and can lay good foundation for production of ethanol fuelby fermentation. |
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| Keywords: | Trichoderma viride Straw stalk Response surface methodology Glucose |
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